EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5345

Fgfr4 fibroblast growth factor receptor 4 ( MGI:95525)
TS16 (10.5 dpc)
in situ hybridisation

Data Images


Fig1D. Copyright: This image is from Li C; Scott DA; Hatch E; Tian X; Mansour SL, Development 2007;134(1):167-76 [doi:10.1242/10.1242/dev.02701] and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:17164422]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: so - somites.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5345_wholemount__moderate.wlz

5345_wholemount__notDetected.wlz

5345_wholemount__strong.wlz

5345_wholemount__possible.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5345_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk somite

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Fgfr4 probeA
Entity Detected:	Fgfr4, fibroblast growth factor receptor 4 ( MGI:95525)
Sequence:	sense strand is shown >Fgfr4 probeA CTCGAGCCAAGCCCCTTCCCTTTCTCTGACTCGCAGACGACATGAGCCGGGGAGCAGCAATGTTGTATGG GCTACGCGGCCCATGGCCGTGGGTCTCCTCGCTGAGCTGCAACCTGATGCATCGACATTTAATGTTGGCA GTGTCAGGCCTCTGACTTGAGACTACTGCTGTCGCAGATCCTCTCTCTGGCCCTGTTTTGGGGAGGGCCA TTCTTGGTCCTAAGGTTCATAGTTGAGGCCTTCTGTTCCAGCCTTATGCTCCCATCTCAGAGTTCAACTC TCATCTCAAGATCATGGCCTTGCCCTTGGACTCATCCTCAGAGAAGTTAAGCATTAAGGCCTTGGCACGC AGCCTCCGTCTCCGGGGCTCTCCGGGACTAGCTGCAAAACTTATGCTCTAAACATTTCTAGTTCCCCCAA ACAACCTAGAGGCCTTGGGACTTCACATCCCCCAGCACACAAGCCTCACCACCCCCTGCCATCCCCCCTC CATTGCTTGTTCCAGCATCTTGGTGAAAGGGGCATCAGCTCTGGTGTCCCTGAGAGACGAGAAGCCTGTG GGAACGACAGAAGAACATGGCATTTTTATAAATTA
	nt 2697 - nt 3291 of XM_193720.2
Notes:	The Fgfr4 probe used in this study by Li et al., 2007 [PMID:17164422] is indicated as that previously described by Wright et al., 2003 [PMID:14517998] i.e: "The Fgfr4 probe contained 700 bp from the 3' untranslated region (mFgfr4, accession no. XM193720, bp2697-3291). An antisense probe was generated by digestion with XhoI, followed by transcription with T7 RNA polymerase." Editors note: There are two versions of XM_193720. The sequences contain 3299nt of identical sequence and differ only by XM_193720.2 (3323bp) extending for a further 24bp at the 3' end. A XhoI site is found at position 2697 of either sequence. However, a fragment extending between nt "2697-3291" would be 594nt in length and not 700nt as indicated by the authors.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS16
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Li C; Scott DA; Hatch E; Tian X; Mansour SL, 2007 [PMID:17164422] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.02701] [ PMID:17164422] Li C, Scott DA, Hatch E, Tian X, Mansour SL 2007 Dusp6 (Mkp3) is a negative feedback regulator of FGF-stimulated ERK signaling during mouse development. Development (134):167-76
	[ doi:10.1002/dvdy.10362] [ PMID:14517998] Wright TJ, Hatch EP, Karabagli H, Karabagli P, Schoenwolf GC, Mansour SL 2003 Expression of mouse fibroblast growth factor and fibroblast growth factor receptor genes during early inner ear development. Dev Dyn (228):267-72
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source