EMAGE mouse gene expression spatial database

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EMAGE:5373

Gjd2 gap junction protein, delta 2 ( MGI:1334209)
TS16 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2C.Gulisano M; Parenti R; Spinella F; Cicirata F, "Cx36 is dynamically expressed during early development of mouse brain and nervous system." Neuroreport 2000;11: 3823-8. [PMID:11117498]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5373_wholemount__possible.wlz

5373_wholemount__notDetected.wlz

5373_wholemount__strong.wlz

5373_wholemount__moderate.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5373_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future brain

detected

regional

Expression restricted to anterior territory of the brain and essentially in the neuroepithelium.

diencephalon

detected

regional

Note that one day earlier, the posterior border of expression corresponded to the area where the zona limitans intrathalamica (ZLI) will form.

optic stalk

detected

optic cup

detected

future midbrain

detected

regional

The caudal margin of the expression domain is in the pretectum area. The signal appears to be homogeneous throughout the marked region. Note that one day earlier, the posterior border of expression corresponded to the area where the zona limitans intrathalamica (ZLI) will form.

telencephalon

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Gjd2 probeA
Entity Detected:	Gjd2, gap junction protein, delta 2 ( MGI:1334209)
Notes:	The Gjd2 (Cx36) probe used in this study by Gulisano et al., 2000 [PMID:11117498] was "generated from a genomic fragment corresponding to intracellular loop of the mouse C�36 gene Cicirata et al., 2000 [PMID:10876089] ". Editors Note: Cicirata et al. describe a probe used to isolate genomic clones which "was a 302 bp PCR fragment encoding the intracellular loop of the mCx36 sequence (position 283-585 in the mouse sequence), subcloned in pBluescript KS+". It is not specified by Cirata et al whether the template for this probe was cDNA or genomic DNA, neither do they refer the co-ordinates given to a particular sequence (two sequences are shown in the paper and the authors also refer to AF226992 - each of which use different numbering conventions). There is insufficient information to define the sequence.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	10.5 dpc
Theiler Stage:	TS16
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Gulisano M; Parenti R; Spinella F; Cicirata F, 2000 [PMID:11117498] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1097/00001756-200011270-00045] [ PMID:11117498] Gulisano M, Parenti R, Spinella F, Cicirata F 2000 Cx36 is dynamically expressed during early development of mouse brain and nervous system. Neuroreport (11):3823-8
	[ doi:10.1016/S0378-1119(00)00202-X] [ PMID:10876089] Cicirata F, Parenti R, Spinella F, Giglio S, Tuorto F, Zuffardi O, Gulisano M 2000 Genomic organization and chromosomal localization of the mouse Connexin36 (mCx36) gene. Gene (251):123-30
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source