EMAGE mouse gene expression spatial database

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EMAGE:5377

Gpc3 glypican 3 ( MGI:104903)
TS17 (10.5 dpc)
in situ hybridisation

Data Images


Figure 2C. [doi:10.1002/(SICI)1097-0177(199812)213:4<431::AID-AJA8>3.0.CO;2-7] Copyright: This image is from Pellegrini M; Pilia G; Pantano S; Lucchini F; Uda M; Fumi M; Cao A; Schlessinger D; Forabosco A, "Gpc3 expression correlates with the phenotype of the Simpson-Golabi-Behmel syndrome." Dev Dyn 1998; 213: 431-9. Reprinted with permission of Wiley-Liss Inc. [PMID:9853964] .	Figure 3A. [doi:10.1002/(SICI)1097-0177(199812)213:4<431::AID-AJA8>3.0.CO;2-7] Copyright: This image is from Pellegrini M; Pilia G; Pantano S; Lucchini F; Uda M; Fumi M; Cao A; Schlessinger D; Forabosco A, "Gpc3 expression correlates with the phenotype of the Simpson-Golabi-Behmel syndrome." Dev Dyn 1998; 213: 431-9. Reprinted with permission of Wiley-Liss Inc. [PMID:9853964] .	Figure 3B. [doi:10.1002/(SICI)1097-0177(199812)213:4<431::AID-AJA8>3.0.CO;2-7] Copyright: This image is from Pellegrini M; Pilia G; Pantano S; Lucchini F; Uda M; Fumi M; Cao A; Schlessinger D; Forabosco A, "Gpc3 expression correlates with the phenotype of the Simpson-Golabi-Behmel syndrome." Dev Dyn 1998; 213: 431-9. Reprinted with permission of Wiley-Liss Inc. [PMID:9853964] .

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Fig2A shows a lateral view of wholemount stained 10.5dpc embryo. Figs3A and B show transverse sections of a 10.5dpc embryo after wholemount ISH. 3A shows the expression at the level of the maxillary and mandibular components of the first branchial arch, whereas 3B shows Gpc3 signals in the dermamyotome and sclerotome components of somites and no signals in the neural tube. Image annotations: in Fig2A: first (I) and second (II) branchial arches; limb buds (arrows), in Fig3A: mandibular (md) and maxillary (mx) components of the 1st branchial arch, in Fig3B: neural tube (nt), somintes (so).

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

5377_wholemount__moderate.wlz

5377_wholemount__weak.wlz

5377_wholemount__possible.wlz

5377_wholemount__notDetected.wlz

5377_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5377_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

2nd branchial arch

strong

There is a particularly intense signal in the second branchial arch.

mesenchyme

weak

Diffuse expression in mesenchymal tissues.

trunk somite

strong

regional

Intense expression is detected in dermamyotome and sclerotome.

neural tube

not detected

homogeneous

1st branchial arch mandibular component mesenchyme

strong

There is a particularly intense signal in the head mesenchyme contributing to the maxillary and mandibulary components of the first branchial arch.

1st branchial arch maxillary component mesenchyme

strong

There is a particularly intense signal in the head mesenchyme contributing to the maxillary and mandibulary components of the first branchial arch.

forelimb bud

strong

There is a particularly intense signal in the anterior and posterior limb buds.

hindlimb bud

strong

There is a particularly intense signal in the anterior and posterior limb buds.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Gpc3 probeB
Entity Detected:	Gpc3, glypican 3 ( MGI:104903)
Sequence:	sense strand is shown >Gpc3 probeB TCTTTCCAGTCATCTACACCCAGATGATGAACCCAGGCCTGCCTGAGTCAGTCTTAGACATCAACGAGTG CCTCCGAGGAGCAAGACGTGACCTGAAAGTATTTGGCAGTTTCCCCAAGCTTATTATGACCCAGGTTTCC AAGTCACTGCAAGTCACTCGAATCTTCCTTCAAGCCCTGAATCTCGGAATTGAAGTCATCAACACTACCG ACCACCTCAAGTTTAGTAAGGACTGTGGCCGTATGCTCACCCGAATGTGGTATTGCTCTTACTGCCAGGG ACTGATGATGGTTAAGCCTTGCGGTGGTTATTGCAATGTGGTCATGCAAGGCTGTATGGCTGGTGTGGTG GAGATCGACAAGTACTGGAGAGAATACATTCTGTCTCTTGAAGAGCTCGTGAATGGCATGTACAGAATCT ACGACATGGAGAATGTGCTGCTCGGCCTCTTTTCTACCATCCATGATTCCATCCAGTATGTGCAGAAGAA CGGAGGCAAGCTGACCACCACCATTGGCAAGTTGTGTGCCCACTCCCAGCAACGCCAATATAGATCTGCT TATTACCCTGAAGATCTGTTTATTGACAAGAAGATATTAAAAGTCGCTCATGTCGAACATGAAGAAACCT TATCCAGCCGAAGAAGGGAACTGATTCAGAAACTGAAGTCTTTCATCAACTTCTATAGCGCTTTGCCGGG CTACATCTGCAGCCATAGCCCCGTGGCCGAAAATGATACCCTGTGCTGGAACGGACAAGAAC
	nt 635 - nt 1396 of NM_016697.2
Notes:	The Gpc3 probe template used in this study by Pellegrini et al., 1998 [PMID:9853964] was generated using "primers derived from the human cDNA" where "an equivalent portion was recovered from a mouse embryonic library ("5'-stretch" from Clontech, Palo Alto, CA) by PCR amplification. The primers, which bear an EcoRI and XbaI site, respectively, at their 5' and 3' ends to facilitate cloning of the product, were CGGAATTCTTTCCAGTCATCTATACCC and GCTCTACACGAGTTCTTGTCCATTC. They amplify a 763-bp product (SGB.2) from the cDNA mixture."
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	10.5 dpc
Theiler Stage:	TS17
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Pellegrini M; Pilia G; Pantano S; Lucchini F; Uda M; Fumi M; Cao A; Schlessinger D; Forabosco A, 1998 [PMID:9853964] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1002/(SICI)1097-0177(199812)213:4<431::AID-AJA8>3.0.CO;2-7] [ PMID:9853964] Pellegrini M, Pilia G, Pantano S, Lucchini F, Uda M, Fumi M, Cao A, Schlessinger D, Forabosco A 1998 Gpc3 expression correlates with the phenotype of the Simpson-Golabi-Behmel syndrome. Dev Dyn (213):431-9
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source