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EMAGE:5382

Grlf1 glucocorticoid receptor DNA binding factor 1 ( MGI:1929494)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:5382
Figure 7J. Copyright: This image is from Brouns MR; Matheson SF; Hu K; Delalle I; Caviness VS; Silver J; Bronson RT; Settleman J, Development 2000; 127: 4891-903 and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:11044403]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: arrowhead indicates the developing eye.
Expression Pattern Description
Spatial Annotation:
EMAGE:5382Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5382_wholemount__moderate.wlz
5382_wholemount__weak.wlz
5382_wholemount__notDetected.wlz
5382_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5382_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
eye
detected detected
embryo
detected detected
Expression appears more widespread than at a slightly earlier (7.5dpc) stage.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Grlf1 probeA
Entity Detected:Grlf1, glucocorticoid receptor DNA binding factor 1 ( MGI:1929494)
Notes:The Grlf1 (p190 RhoGAP) probe template used in this study by Brouns et al., 2000 [PMID:11044403] was "pBluescript SK +/- plasmid containing p190 RhoGAP coding sequence (bp 1694-3610)". Editor's note: the authors do not refer these co-ordinates to a specific sequence therefore the probe sequence is unreported. However, the probe does not cover the entire coding sequence as according to the Grlf1 cDNA RefSeq NM_172739.4 the coding region is 4499 bp in length (over twice the length of the probe used here).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Brouns MR; Matheson SF; Hu K; Delalle I; Caviness VS; Silver J; Bronson RT; Settleman J, 2000 [PMID:11044403] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11044403] Brouns MR, Matheson SF, Hu KQ, Delalle I, Caviness VS, Silver J, Bronson RT, Settleman J 2000 The adhesion signaling molecule p190 RhoGAP is required for morphogenetic processes in neural development. Development (127):4891-903
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE