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EMAGE:5386

H3f3a H3 histone, family 3A ( MGI:1097686)
TS17 (11.5 dpc)
in situ reporter

Data Images
EMAGE:5386
Figure 3 11.5. Couldrey C; Carlton MB; Nolan PM; Colledge WH; Evans MJ, "A retroviral gene trap insertion into the histone 3.3A gene causes partial neonatal lethality, stunted growth, neuromuscular deficits and male sub-fertility in transgenic mice." Hum Mol Genet 1999; 8: 2489-95. [PMID:10556297]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5386Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5386_wholemount__moderate.wlz
5386_wholemount__weak.wlz
5386_wholemount__possible.wlz
5386_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5386_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalWidespread embryonic expression was seen from 8.5 to 10.5dpc - at this stage (11.5dpc) expression became somewhat more restricted.
limb
detected detected
regionalIntense staining on apical surface of developing limbs.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:H3f3a, H3 histone, family 3A ( MGI:1097686)
Notes:H3f3a (H3.3A) expression was detected in this study by Couldrey et al., 1999 [PMID:10556297] in embryos heterozygous for a gene trapped allele, where the ROSA-betageo retroviral gene trap vector containing a lacZ/neo fusion gene integrated into the first intron of the gene. Although not detectable by Northern analysis, detection of transcript by RT-PCR indicated that the mutation is a hypomorph.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129/Sv
Age:11.5 dpc
Theiler Stage:TS17
Mutations:H3f3aGt(ROSABetageo)14E7Ev/H3f3a+
Preparation:wholemount
Procedures
Fixation:0.4% paraformaldehyde
General Information
Authors:Couldrey C; Carlton MB; Nolan PM; Colledge WH; Evans MJ, 1999 [PMID:10556297] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/hmg/8.13.2489] [ PMID:10556297] Couldrey C, Carlton MB, Nolan PM, Colledge WH, Evans MJ 1999 A retroviral gene trap insertion into the histone 3.3A gene causes partial neonatal lethality, stunted growth, neuromuscular deficits and male sub-fertility in transgenic mice. Hum Mol Genet (8):2489-95
Links:MGI:2682960 same experiment
 MGI:2682911 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI