EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5393

Gsc goosecoid homeobox ( MGI:95841)
TS22 (14.5 dpc)
in situ hybridisation

Data Images


Raw signal output, as movie.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

Photograph prior to 3D imaging.	Photograph prior to 3D imaging.	Sample morphology following 3D imaging, as movie

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

palatal shelf

possible

regional

Posterior unfused regions.

premaxilla

not detected

homogeneous

upper lip

not detected

homogeneous

Annotation Validation:

Submitter + EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Gsc_cloneA
Entity Detected:	Gsc, goosecoid homeobox ( MGI:95841)
Sequence:	sense strand is shown >Gsc_cloneA GGTCGGCAGCTCCCGCCTGGGCTACAACAGCTACTTCTACGGGCAGCTGCACGTGCAGGCGGCGCCCGTG GGCCCGGCTTGCTGCGGGGCTGTGCCGCCGCTGGGCGCCCAGCAGTGCTCCTGCGTCCCGACGCCCCCAG GCTACGAGGGCCCCGGTTCTGTACTGGTGTCTCCGGTGCCGCACCAGATGCTGCCCTACATGAACGTGGG CACGCTGTCGCGCACTGAGCTGCAGCTGCTCAACCAGCTGCACTGTCGGCGGAAGCGGCGGCACCGCACC ATCTTCACCGATGAGCAGCTCGAAGCCCTGGAGAACCTCTTCCAGGAGACGAAGTACCCAGACGTGGGCA CTCGGGAGCAGCTGGCCAGGAAGGTGCACCTTCGGGAGGAGAAGGTGGAGGTCTGGTTTAAGAACCGCCG AGCCAAGTGGAGACGACAGAAGCGATCCTCCTCGGAGGAGTCAGAAAACGCCGAGAAGTGGAACAAGACG TCCTCAAAAGCCTCGCCGGAGAAGAGGGAAGAGGAAGGTAAAAGCGATTTGGACTCGGACAGCTGACGGC CGCGGCCGCGGGCCGGCCGGGGCGCTTGCCTGACGCAAGGACTTGCACAGACAGTCGATGCTACTTGCAC ACACCCTGCCTTGCGGGAGGGGGTCGAGAAAGCAACGAGGAGCTAGCTGTAAATAATGTACAGGCCCAGG ACTGCTGGGGACAGGCGCGTGGGGACCGCAGCCCCACTGTCCCGGGCTGCCTGCGTGAGCCTCGTCCCCC ACCGTAGTATTTATAGTTAAGTTAACGGTG
	nt 337 - nt 1136 of NM_010351.1
Notes:	Full length Gsc cDNA was cloned into a plasmid vector. The antisense probe was made by linearising with Sac I and transcribing with T3 RNA polymerase. Plasmid available on request.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD-1
Age:	14.5 dpc
Theiler Stage:	TS22
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Bozena Palinkasova, Mike Dixon
Principal investigator:	Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:	Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:	screen
References:	[ PMID:7555718] Yamada G, Mansouri A, Torres M, Stuart ET, Blum M, Schultz M, De Robertis EM, Gruss P 1995 Targeted mutation of the murine goosecoid gene results in craniofacial defects and neonatal death. Development (121):2917-22
	[ doi:10.1007/BF00539011] [ PMID:8597641] Guenet JL, Simon-Chazottes D, de Robertis E, Blum M 1995 The mouse goosecoid gene (Gsc) maps to the telomeric part of mouse chromosome 12. Mamm Genome (1995):816-7
	[ doi:10.1016/0092-8674(92)90632-M] [ PMID:1352187] Blum M, Gaunt SJ, Cho KW, Steinbeisser H, Blumberg B, Bittner D, De Robertis EM 1992 Gastrulation in the mouse: the role of the homeobox gene goosecoid. Cell (69):1097-106
	[ doi:10.1006/geno.1994.1281] [ PMID:7916327] Blum M, De Robertis EM, Kojis T, Heinzmann C, Klisak I, Geissert D, Sparkes RS 1994 Molecular cloning of the human homeobox gene goosecoid (GSC) and mapping of the gene to human chromosome 14q32.1. Genomics (21):388-93
	[ PMID:10433910] Rivera-Perez JA, Wakamiya M, Behringer RR 1999 Goosecoid acts cell autonomously in mesenchyme-derived tissues during craniofacial development. Development (1999):3811-21
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source