Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5396

Hdac1 histone deacetylase 1 ( MGI:108086)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5396
Fig3C. [doi:10.1093/emboj/21.11.2672] Lagger G; O'Carroll D; Rembold M; Khier H; Tischler J; Weitzer G; Schuettengruber B; Hauser C; Brunmeir R; Jenuwein T; Seiser C. EMBO J 2002; 21: 2672-81. "Essential function of histone deacetylase 1 in proliferation control and CDK inhibitor repression." [PMID:12032080] © Nature Publishing Group. Reproduced with permission from EMBO J.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
HDAC1 is highly expressed throughout the E9.5 embryo except in the developing heart (he), with pronounced expression in the branchial arches (ba) and the limb bud (lb).
Expression Pattern Description
Spatial Annotation:
EMAGE:5396Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5396_wholemount__moderate.wlz
5396_wholemount__weak.wlz
5396_wholemount__possible.wlz
5396_wholemount__notDetected.wlz
5396_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5396_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalHighly expressed throughout the embryo except the heart.
heart
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hdac1 probeA
Entity Detected:Hdac1, histone deacetylase 1 ( MGI:108086)
Sequence:sense strand is shown

>Hdac1 probeA
AAGCTTCACATCAGCCCTTCTAACATGACCAACCAGAACACTAACGAGTACCTGGAGAAGATCAAGCAGC
GTCTCTTTGAGAACTTGAGGATGCTGCCCCATGCCCCTGGGGTCCAGATGCAGGCCATCCCTGAGGACGC
CATCCCCGAAGAGAGTGGGGATGAAGATGAGGAGGACCCTGACAAACGCATCTCCATCTGCTCCTCTGAT
AAACGCATTGCCTGTGAGGAAGAGTTCTCGGACTCAGATGAGGAGGGAGAAGGTGGTCGCAAGAACTCTT
CTAACTTCAAAAAAGCCAAAAGAGTTAAAACAGAGGATGAGAAAGAGAAAGATCCTGAAGAGAAAAAAGA
AGTCACAGAAGAAGAGAAAACCAAGGAGGAGAAGCCAGAAGCCAAAGGGGTCAAAGAAGAGGTCAAGTTG
GCCTGAGCAAGGTCTGCAGCCCCATCTTCTCCCCAAGTTCCTCACTCTCCCTCAACTTCTCAGATTTTAT
ATTTTCTATTCCTCTGTGTA
nt 1041 - nt 1550 of X98207.1
Notes:The Hdac1 probe used in this study by Lagger et al., 2002 [PMID:12032080] was a "3' fragment encompassing bp 1041-1550 (Bartl et al., 1997 [PMID:9271381] )", where Bartl et al. provide the full sequence in both Fig2 therein and in the accompanying GenBank entry X98207.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Lagger G; O'Carroll D; Rembold M; Khier H; Tischler J; Weitzer G; Schuettengruber B; Hauser C; Brunmeir R; Jenuwein T; Seiser C, 2002 [PMID:12032080] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/emboj/21.11.2672] [ PMID:12032080] Lagger G, O'Carroll D, Rembold M, Khier H, Tischler J, Weitzer G, Schuettengruber B, Hauser C, Brunmeir R, Jenuwein T, Seiser C 2002 Essential function of histone deacetylase 1 in proliferation control and CDK inhibitor repression. EMBO J (21):2672-81
 [ PMID:9271381] Bartl S, Taplick J, Lagger G, Khier H, Kuchler K, Seiser C 1997 Identification of mouse histone deacetylase 1 as a growth factor-inducible gene. Mol Cell Biol (17):5033-43
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE