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EMAGE:5397

Shox2 short stature homeobox 2 ( MGI:1201673)
TS22 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:5397 EMAGE:5397 EMAGE:5397 EMAGE:5397 EMAGE:5397
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5397 EMAGE:5397 EMAGE:5397
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: one star
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
palatal shelf
strong strong
homogeneousAnterior part only.
upper lip
moderate moderate
regional
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Shox2_cloneA
Entity Detected:Shox2, short stature homeobox 2 ( MGI:1201673)
Sequence:sense strand is shown

>Shox2_cloneA
GTTTGGTTTCAAAATCGAAGAGCTAAGTGTAGAAAACAGGAAAATCAACTTCACAAAGGTGTCCTTATAG
GAGCCGCTAGCCAGTTTGAAGCTTGTAGAGTTGCACCCTATGTCAACGTAGGTGCTTTAAGGATGCCATT
TCAGCAGGATAGTCATTGCAACGTGACGCCCTTGTCCTTTCAGGTTCAGGCGCAGCTGCAGCTGGACAGC
GCCGTGGCGCACGCGCACCACCACCTGCATCCGCACCTGGCCGCGCACGCGCCTTACATGATGTTCCCGG
CACCGCCCTTCGGACTGCCGCTGGCCACGCTGGCCGCGGACTCGGCCTCGGCCGCCTCGGTGGTGGCCGC
TGCCGCCGCCGCCAAGACCACCAGCAAGAACTCCAGCATCGCGGATCTCAGACTGAAAGCTAAAAAGCAC
GCGGCCGCCCTGGGTCTGTGACGCCGGCGCCA
nt 771 - nt 1222 of NM_013665.1
Notes:The Shox2 probe fragment (insert size: about 0.6kb) was cloned into the pBS-KS cloning vector. The antisense probe was generated by cutting with XhoI restriction enzyme and transcribing with the T7 polymerase. Probe available upon request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:14.5 dpc
Theiler Stage:TS22
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1242/dev.02013] [ PMID:16141225] Yu L, Gu S, Alappat S, Song Y, Yan M, Zhang X, Zhang G, Jiang Y, Zhang Z, Zhang Y, Chen Y 2005 Shox2-deficient mice exhibit a rare type of incomplete clefting of the secondary palate. Development (132):4397-406
 [ doi:10.1387/ijdb.062212ql] [ PMID:17294368] Li Q, Ding J 2007 Gene expression analysis reveals that formation of the mouse anterior secondary palate involves recruitment of cells from the posterior side. Int J Dev Biol (51):167-72
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE