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EMAGE:5398

Hdac5 histone deacetylase 5 ( MGI:1333784)
TS17 (10.5 dpc)
in situ reporter

Data Images
EMAGE:5398 EMAGE:5398
Fig2_upper_E10.5 of [doi:10.1128/MCB.24.19.8467-8476.2004] Mol Cell Biol 2004; 24: 8467-76 [PMID:15367668] Chang S; McKinsey TA; Zhang CL; Richardson JA; Hill JA; Olson EN, "Histone deacetylases 5 and 9 govern responsiveness of the heart to a subset of stress signals and play redundant roles in heart development." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2001. Fig2_lower_E10.5 of [doi:10.1128/MCB.24.19.8467-8476.2004] Mol Cell Biol 2004; 24: 8467-76 [PMID:15367668] Chang S; McKinsey TA; Zhang CL; Richardson JA; Hill JA; Olson EN, "Histone deacetylases 5 and 9 govern responsiveness of the heart to a subset of stress signals and play redundant roles in heart development." © ASM. Reproduced with permission from Molecular and Cellular Biology. Reuse of any Licensed Material requires permission from ASM. Copyright 2001.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The section is a transverse section through LacZ-stained embryos visualised in bright field, with LacZ staining indicated in pink. h, heart. The authors state that the expression pattern of beta-galactosidase during pre- and postnatal development reflected that of the endogenous HDAC5 gene (data not shown).
Expression Pattern Description
Spatial Annotation:
EMAGE:5398Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5398_wholemount__moderate.wlz
5398_wholemount__weak.wlz
5398_wholemount__notDetected.wlz
5398_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5398_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
strong strong
future spinal cord
strong strong
regionalExpressed ventrally.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Hdac5, histone deacetylase 5 ( MGI:1333784)
Notes:Hdac5 expression was revealed in this study by Chang et al., 2004 [PMID:15367668] in embryos heterozygous for a targeted allele where the targeting vector was designed to replace exons 3-7 with a lacZ-neo cassette. The deleted sequence encoded residues 32-298, including the region responsible for interaction with MEF2. RT-PCR and sequencing revealed that exon 2 was spliced to the 5' end of exon 3, which was spliced to the lacZ coding sequence. Transcripts of the wild-type were not found in hearts from mutants. Staining indicated that lacZ expression reflected that of the endogenous gene during pre- and postnatal development.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129S2/SvPas * C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:Hdac5tm1Eno/Hdac5+
Preparation:wholemount
Procedures
General Information
Authors:Chang S; McKinsey TA; Zhang CL; Richardson JA; Hill JA; Olson EN, 2004 [PMID:15367668] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1128/MCB.24.19.8467-8476.2004] [ PMID:15367668] Chang S, McKinsey TA, Zhang CL, Richardson JA, Hill JA, Olson EN 2004 Histone deacetylases 5 and 9 govern responsiveness of the heart to a subset of stress signals and play redundant roles in heart development. Mol Cell Biol (24):8467-76
Links:MGI:3056065 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE