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EMAGE:5415

Hand1 heart and neural crest derivatives expressed transcript 1 ( MGI:103577)
TS22 (14.0 dpc)
in situ hybridisation

Data Images
EMAGE:5415 EMAGE:5415 EMAGE:5415 EMAGE:5415 EMAGE:5415
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5415 EMAGE:5415 EMAGE:5415 EMAGE:5415
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: one star
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
upper lip
moderate moderate
regional
palatal shelf
strong strong
regional
primary palate
possible possible
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hand1_probeA
Entity Detected:Hand1, heart and neural crest derivatives expressed transcript 1 ( MGI:103577)
Sequence:sense strand is shown

>Hand1_probeA
AGATGGGACTGGAGAAGACCAAGCCCCCGAAGACACACCCTTCCAACCCATCTGGGTGGAACTCAACCCC
AAAAGCCATGGTCGCGCCGCTCTCTGGCTCGCTCTCTCGTCCCCTTCTTGGCTCTGCGTCAGTACCCTGA
TGCCTTCTCGAGTTAAAATGCAATAAGAGTAATTGGTAGTTTCTAACCAGGAAGACTGACGCTGCCTGGG
TTGACCTCTCCTGAGCAGCGATGGTTTTCCTTCTTGTCGCTACACTTCCTACCTAGAGGGAAGGCGTTGC
TCCCTTGCGCAGCACCTCTGCTTTCCAAGGGAAATCTTTCCCTTCCTTTCCCTTCCCTTCCCTCCCCTCT
CCTCCTCCTTTCCCCTCTTCCGTCCTCTTACCTCCTCTTTCTCTTCCTTCCTTCTCCTTCATTTCTTTCC
TTTTCCTTCTAAATAAAAGCGTTCCCATTCGTTGCTGAATTTGTAACGTATTTAAACTACCTATCATGCC
TGTTGCTCTCAGGTATACATCTCCCTACCCCCACCCTCTTTCTACCTCAAATCAGTGTGACCACTCGCAG
CCCCACCCTTTGCCAAAGCAGTATCTATGCTCTTGACTTAATAAAAAGTTTTCAGAG
nt 1282 - nt 1898 of NM_008213.2
Notes:A probe template fragment of Hand1 (insert size 1.9kb) was cloned in Bluescript SK II+. To generate the antisense probe the plasmid was cut with NotI and transcribed with T7 RNA polymerase. Probe available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:14.0 dpc
Theiler Stage:TS22
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Bozena Palinkasova, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Bozena Palinkasova, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
References:[ doi:10.1016/j.ydbio.2007.07.036] [ PMID:17764670] Barbosa AC, Funato N, Chapman S, McKee MD, Richardson JA, Olson EN, Yanagisawa H 2007 Hand transcription factors cooperatively regulate development of the distal midline mesenchyme. Dev Biol (310):154-68
 [ doi:10.1242/dev.01091] [ PMID:15073150] Morikawa Y, Cserjesi P 2004 Extra-embryonic vasculature development is regulated by the transcription factor HAND1. Development (131):2195-204
 [ doi:10.1016/j.mod.2004.04.012] [ PMID:15172687] Huang F, Wagner M, Siddiqui MA 2004 Ablation of the CLP-1 gene leads to down-regulation of the HAND1 gene and abnormality of the left ventricle of the heart and fetal death. Mech Dev (121):559-72
 [ doi:10.1038/ng0398-266] [ PMID:9500550] Firulli AB, McFadden DG, Lin Q, Srivastava D, Olson EN 1998 Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1. Nat Genet (18):266-70
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE