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EMAGE:5422

Tbx22 T-box 22 ( MGI:2389465)
TS21 (13.5 dpc)
in situ hybridisation

Data Images
EMAGE:5422 EMAGE:5422 EMAGE:5422 EMAGE:5422 EMAGE:5422
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging. Tbx22 expression at E13.5 is restricted to the posterior edges of the palatal shelves.
EMAGE:5422
Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
palatal shelf
strong strong
regionalTbx22 expression at E13.5 is restricted to the posterior edges of the palatal shelves.
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Tbx22_cloneA
Entity Detected:Tbx22, T-box 22 ( MGI:2389465)
Sequence:sense strand is shown

>Tbx22_cloneA
GAATTCACTACTGTGACAGCTTACCAAAACCAGCAGATTACCAAACTGAAAATAGACAGGAATCCTTTTG
CCAAAGGATTTAGAGATCCTGGGAGAAATAGGGGTGTCTTAGATGGATTTTTAGAGACCTACCCATGGAT
GCCTTCTTTCTCTATGGATTTTAAAACTTTTGTCACAGACACACAAAGTGGAAGCAGTGGCTCATCTCCA
GTGACCTCTAGTGGAGGAGCTCCCTCTCCTTTGAACTCCTTACTTTCTCCATCTTGTTCTCCACCTATGG
TTTACATACCTCCAAGCTCCTTTGGGATGACCTATCCAGATGCATACCTGCACAGTGTCAACATACCCTT
CTGCTACAGAATTTGTCCAACTAATAATTGGCGATCACAACCTTTTGTTTTACCTACTCCTGAAAGGCTA
CCAAGTTTCATCATTCCTCAGACCTTACCCCCACTCATGATGGAAGTCCCAGTGGTATCCTCCAGGGGCA
TCATCAATCCAAACAGTGGCTTACATGAAGACTGCAATGGGCAGTGTCTACAAGCATCTCATTCTGCCAA
TCAAATGTTATATGGATTACAGAATCCTGGAAACATTTTCCATCCAAACGCCATTGCCCAAGAAGCAA
nt 1168 - nt 541 of BC152402.1
Notes:The Tbx22 probe fragment (~600bp) cloned into pBS was kindly donated by Rulang Jiang. To produce the antisense probe the template plasmid was cut with BamHI and transcription performed with the T3 polymerase. Probe available on request.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:13.5 dpc
Theiler Stage:TS21
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Laura Ward, Mike Dixon
Principal investigator:Mike Dixon, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Submitted by:Laura Ward, University of Manchester, Faculty of Life Sciences Michael Smith Building Oxford Road, Manchester, England M13 9PT
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE