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EMAGE:547

Wnt3 wingless-related MMTV integration site 3 ( MGI:98955)
TS09 (6.5 dpc)
in situ hybridisation

Data Images
EMAGE:547 EMAGE:547
Figure 7B of Perea-Gomez et al., 2001 [PMID:11171400] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 7A of Perea-Gomez et al., 2001 [PMID:11171400] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
A is a brightfield image of an adjacent section to B.
Expression Pattern Description
Spatial Annotation:
EMAGE:547EMAGE:547Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
547_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:547_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo ectoderm
detected detected
regionalExpression is detected in the posterior epiblast and mesodermal cells
embryo mesoderm
detected detected
regionalExpression is detected in the posterior epiblast and mesodermal cells
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2182553
Entity Detected:Wnt3, wingless-related MMTV integration site 3 ( MGI:98955)
Notes:The Wnt3 probe used in this study by Perea-Gomez et al., 2001 [PMID:11171400] is indicated as being previously described by Liu et al., 1999 [PMID:10431240] ie. as a "0.8-kb cDNA fragment containing exons 3-5 (Roelink et al., 1990 [PMID:2162045] ) ". Roelink et al. refer to three Wnt3 cDNA clones, neither of which are 0.8kb.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:6.5 dpc
Theiler Stage:TS09
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Perea-Gomez et al., 2001 [PMID:11171400] Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171400] Perea-Gomez A, Lawson KA, Rhinn M, Zakin L, Brulet P, Mazan S, Ang SL 2001 Otx2 is required for visceral endoderm movement and for the restriction of posterior signals in the epiblast of the mouse embryo. Development (128):753-65
 [ doi:10.1038/11932] [ PMID:10431240] Liu P, Wakamiya M, Shea MJ, Albrecht U, Behringer RR, Bradley A 1999 Requirement for Wnt3 in vertebrate axis formation. Nat Genet (22):361-5
 [ doi:10.1073/pnas.87.12.4519] [ PMID:2162045] Roelink H, Wagenaar E, Lopes da Silva S, Nusse R 1990 Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain. Proc Natl Acad Sci U S A (87):4519-23
Links:MGI:1930293 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI