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EMAGE:5486

Cldn4 claudin 4 ( MGI:1313314)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:5486 EMAGE:5486
Scale bar is 200 microns. Lateral view, anterior left Scale bar is 200 microns Anterior view

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5486Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5486_wholemount_moderate_3D_1.wlz
5486_wholemount_notDetected_3D_1.wlz
5486_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5486_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalSubmitter describes expression as regionally restricted to the "endoderm" and "various other tissues".
embryo endoderm
detected detected
Submitter describes expression as regionally restricted to the "endoderm".
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1030499
Entity Detected:Cldn4, claudin 4 ( MGI:1313314)
Sequence:sense strand is shown

>MGI:1030499
GCCCAGGACAGGGGCCTCTGAACGCCTTTGTTGGCCCAAGCTCAGGAATCCAGAGAAACTGGTCCCGAGG
GAGGCCTCCGGGACTGAAACCCCTCCCTCTGCCTCCGCCCCTCCTCTAGGAGCTTTAACTGCAGCACCTG
TGGCCGCCCTTTCTTGTTGCAGGTGCAGGCAGGAAAGAGCTGCAGTGTTCGCGCTTGGTAGCTGGTGCAT
CGGACTCAGCTGGCTTTGTGTCCCTGAGGCTCACCGAAAAAAACTTTCTCAGCCCTCTGACTCCAGAGAG
AGAGAGAGAGAGGTACTTTTTGTGGTCACCGACTTTGACCCCTGCAGAGGCTGAGCGATGGCGTCTATGG
GACTACAGGTCCTGGGAATCTCCTTGGCAGTCCTGGGCTGGCTGGGGATCATCCTGAGTTGTGCGCTCCC
CATGTGGCGGGTGACCGCCTTCATCGGCAGCAACATAGTCACAGCACAGACCAGCTGGGAGGGCCTCTGG
ATGAACTGCGTGGTGCAGAGCACACGTCACATGCAGTGCAAGATGTACGACTCGATGCTCGCCCTGCCAG
CAGACCTGCAGGCCGACCGAGCCCTTATTGTCATTAACATCATCTACGGTGCTCATGGGATGCTTCTCTC
A
Notes:Submitter's Information: The probe was made from the entire insert sequence of the IMAGE:2650047 clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE