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EMAGE:5495

Col1a1 collagen, type I, alpha 1 ( MGI:88467)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:5495 EMAGE:5495 EMAGE:5495 EMAGE:5495 EMAGE:5495
Specimen A.Scale bar is 200 microns. Lateral view, anterior left Specimen A.Scale bar is 200 microns. Anterior view Specimen A.Scale bar is 200 microns. Posterior view Specimen B.Scale bar is 200 microns. Anterior view Specimen B.Scale bar is 200 microns. Lateral view, anterior left
EMAGE:5495
Specimen B.Scale bar is 200 microns. Posterior view

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: There are two specimens, both TS11 shown in this entry.
Expression Pattern Description
Spatial Annotation:
EMAGE:5495Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5495_wholemount_moderate_3D_1.wlz
5495_wholemount_weak_3D_1.wlz
5495_wholemount_notDetected_3D_1.wlz
5495_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5495_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
extraembryonic component
detected detected
regionalThe submitter describes the expression as being regionally specific in various tissues.
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3069107
Entity Detected:Col1a1, collagen, type I, alpha 1 ( MGI:88467)
Sequence:sense strand is shown

>MGI:3069107
GGTGATGCTGGTCCCAAAGGTGCTGATGGTTCTCCTGGTAAAGATGGTGCCCGTGGTCTGACTGGTCCCA
TTGGTCCTCCTGGCCCTGCTGGTGCCCCCGGTGACAAGGGTGAAGCTGGTCCCAGTGGTCCTCCCTTTCC
CACCGGAGCCCGTGGTGCTCCCGGAGACCGTGGTGAGGCTGGTCCCCCTGGTCCTGCTGGCTTTGCCGGC
CCCCCTGGTGCTGATGGCCAACCTGGTGCGAAAGGTGAACCTGGTGATACTGGTGTTAAAGGTGATGCTG
GTCCTCCTGGCCCTGCTGGTCCTGCTGGACCCCCCGGCCCCATTGGTAACGTTGGTGCTCCTGGACCCAA
AGGTCCTCGTGGTGCTGCTGGTCCCCCTGGTGCTACTGGCTTCCCTGGTGCTGCTGGCCGTGTCGGTCCC
CCTGGTCCCTCTGGAAATGCTGGACCCCCTGGCCCTCCCGGTCCCGTTGGCAAAGAAGGGGGCAAAGGTC
CCCGTGGTGAGACTGGCCCTGCTGGACGTCCTGGTGAAGTTGGTCCCCCAGGTCCCCCCGGTCCTGCTGG
TGAGAAAGGATCTCCTGGTGCTGATGGACCTGCTGGC
Notes:Submitter information: H3119H03 is from NIA Mouse 15K cDNA Clone Set. The probe was made from the entire insert sequence of the clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE