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EMAGE:550

Cfc1 cripto, FRL-1, cryptic family 1 ( MGI:109448)
TS11 (late headfold Downs & Davies)
in situ hybridisation

Data Images
EMAGE:550 EMAGE:550 EMAGE:550
Figure 9I of Shen et al., 1997 [PMID:9053319] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 9A of Shen et al., 1997 [PMID:9053319] . The approximate section taken in Figures H and I are shown here. Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 9H of Shen et al., 1997 [PMID:9053319] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
I is a high power image of H. The cartoon in A shows the plane of section in I/H. Arrowhead in H and I: prospective floor plate; arrow in H: posterior lateral plate mesoderm; long arrow in I: splanchnopleure; short arrow in I: visceral yolk sac mesoderm and the somatopleure.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo mesoderm
detected detected
regionalCryptic is expressed by both the somatopleural and splanchnopleural layers of the lateral plate mesoderm, flanking the coelomic cavity. Additionally, Cryptic is specifically expressed by the anterior lateral plate mesoderm
neural ectoderm
detected detected
regionalExpression in the prospective floor plate is also observed in transverse sections through the head folds.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334279
Entity Detected:Cfc1, cripto, FRL-1, cryptic family 1 ( MGI:109448)
Sequence:sense strand is shown

>MGI:1334279
CAGGACTGTATAGGGTCAGCACTTCCAGCCTGGTGGTTCAGAGCTCCTGACCTGAGAGGGCTTCAACACC
TGGACTCCAGGATCTTCCTTTAACCCTGCTGTCTCTGGTCCAGGCAGAGGGCAGAGACATCTTCATCTTG
CAAGACTGTGCATCCTGTAACCTGCTATAGTGATTCCAAGACCTGGAGTAAAGGGTGCCTCCGGGGCTAG
GATATTTGAGTTTCAACTTCTGTGGTCATCGATCCCCAAGCACAGATGAGAGCGAACTCACCAACCCAGG
GTATCAGTTTGAAAATGCATCAAGCCAGGCCTCTGTTTTTGGTGACTGTCGCGCTGCAGCTCATCGGTCT
GGGATACAGTTATCAGAGCGAAGGAGATGGTGCCAGAGAAGTCAGCAATATCCTCTCTCCAGTGATCCCC
GGGACGACACTGGACAGAACTCTGAGTAATTCCAGCAGAAAGAATGACATTCCGGAGGGGGCGCGCCTAT
GGGATTCCCTTCCTGACTCCAGCACTTTGGGAGAGAGTGCAGTCCCTGTATCCCGCTGTTGCCACAATGG
CGGCACCTGTGTTCTGGGCAGTTTCTGCGTGTGCCCTGCCTATTTCACTGGTCGCTATTGCGAGCACGAC
CAGAGGCGCAGAGACTGTGGTGCCCTAGGGCATGGAGCTTGGACCCTGCACAGCTGCCGCCTATGCAGGT
GCATCTTCTCAGCCCTGTACTGCCTCCCACACCAAACGTTCAGCCACTGTGACCTGAAAAGCTTCCTTTC
TTCAGGCGCCAGAGGATCAAGAGAATGCAGCATCCCAAGCCTCCTCCTGCTGGTGCTCTGCCTCCTCCTG
CAGGGTGTGGCTGGTAAGGGCTGAGGCTCCTAAGTGCGATGATAGACTCTCCTCCTGAGCTGTCACCCTT
GATTACACCACAGTGTGCCAGCAAGAAAGCTGGGTGGTGGGCATCTGACTTGGTGTTGTGTCCTGTAAAT
AACAGATTCACTGGAATATGCTGGATTCTCATGCTGTACAATAAAGAGGCTTAATGGTG
nt 1 - nt 1039 of U57720.1
Notes:The Cfc1 (cryptic) probe used is this study by Shen et al, 1997 [PMID:9053319] is described as the "entire cDNA". The cryptic cDNA sequence U57720.1 includes both UTRs and ORF sequence and was a direct submission to GenBank from Michael Shen.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:late headfold Downs & Davies
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount plus section of wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:alkaline phosphatase + NBT/BCIP in 10%PVA
General Information
Authors:Shen et al., 1997 [PMID:9053319] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9053319] Shen MM, Wang H, Leder P 1997 A differential display strategy identifies Cryptic, a novel EGF-related gene expressed in the axial and lateral mesoderm during mouse gastrulation. Development (124):429-42
 [ PMID:8269852] Downs KM, Davies T 1993 Staging of gastrulating mouse embryos by morphological landmarks in the dissecting microscope. Development (118):1255-66
Links:MGI:1334280 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI