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EMAGE:554

Smad5 MAD homolog 5 (Drosophila) ( MGI:1328787)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:554 EMAGE:554
Figure 6E' of Tremblay et al., 2001 [PMID:11566864] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 6E of Tremblay et al., 2001 [PMID:11566864] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Corresponding bright and darkfield images are shown below. Abbreviations used: al, allantois; am, amnion; ch, chorion; ps, primitive streak; ve, visceral endoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:554EMAGE:554Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
554_voxel_strong_3D_1.wlz
554_voxel_weak_3D_1.wlz
554_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:554_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
allantois
detected detected
homogeneousExpression detected throughout the embryo, allantois, extra-embryonic mesoderm and epiblast and is markedly upregulated compared to E6.5.
chorion mesoderm
detected detected
homogeneousExpression detected throughout the embryo, allantois, extra-embryonic mesoderm and epiblast and is markedly upregulated compared to E6.5.
amnion mesoderm
detected detected
homogeneousExpression detected throughout the embryo, allantois, extra-embryonic mesoderm and epiblast and is markedly upregulated compared to E6.5.
embryo
detected detected
homogeneousExpression detected throughout the embryo, allantois, extra-embryonic mesoderm and epiblast and is markedly upregulated compared to E6.5.
allantois mesoderm
detected detected
homogeneousExpression detected throughout the embryo, allantois, extra-embryonic mesoderm and epiblast and is markedly upregulated compared to E6.5.
yolk sac mesoderm
detected detected
homogeneousExpression detected throughout the embryo, allantois, extra-embryonic mesoderm and epiblast and is markedly upregulated compared to E6.5.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2678346
Entity Detected:Smad5, MAD homolog 5 (Drosophila) ( MGI:1328787)
Sequence:sense strand is shown

>MGI:2678346
GAATTCAATCCACAACACAGCCTTCTGGTTCAGTTCAGGAACCTGAGCCACAATGAACCGCACATGCCAC
AAAACGCCACGTTTCCCGATTCTTTCCACCAACCCAACAACGCTCCTTTCCCCTTATCTCCTAACAGCCC
CTATCCTCCTTCCCCTGCTAGCAGCACATATCCCAACTCCCCAGCAAGCTCTGGACCTGGAAGTCCATTT
CAACTCCCAGCTGACACCCCTCCCCCTGCCTATATGCCACCTGATGATCAGATGGCCCCAGATAATTCCC
AGCCTATGGATACAAGCAGTAACATGATTCCTCAGACCATGCCCAGCATATCCAGCAGAGATGTTCAGCC
TGTCGCCTATGAGGAGCCCAAACACTGGTGTT
nt 1127 - nt 1508 of NM_008541.1
Notes:The probe used in this study by Tremblay et al., 2001 [PMID:11566864] is indicated as "the proline-rich linker domain of Smad5 (Meersseman et al. 1997 [PMID:9076683] )" . Meersseman describe the probe: "proline rich 'variable' domain MusMLP antisense probe was made of a subcloned 378bp long EcoRI-TaqI fragment (of pMLP1.2 - a 1.2kb Madh5 cDNA)"
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:MF1
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Tremblay et al., 2001 [PMID:11566864] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11566864] Tremblay KD, Dunn NR, Robertson EJ 2001 Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development (128):3609-21
 [ doi:10.1016/S0925-4773(96)00629-6] [ PMID:9076683] Meersseman G, Verschueren K, Nelles L, Blumenstock C, Kraft H, Wuytens G, Remacle J, Kozak CA, Tylzanowski P, Niehrs C, Huylebroeck D 1997 The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation. Mech Dev (61):127-40
Links:MGI:2155960 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE