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EMAGE:555

Smad5 MAD homolog 5 (Drosophila) ( MGI:1328787)
TS14 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:555 EMAGE:555
Figure 6F' of Tremblay et al., 2001 [PMID:11566864] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 6F of Tremblay et al., 2001 [PMID:11566864] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Corresponding dark- and bright-field images are shown.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2678346
Entity Detected:Smad5, MAD homolog 5 (Drosophila) ( MGI:1328787)
Sequence:sense strand is shown

>MGI:2678346
GAATTCAATCCACAACACAGCCTTCTGGTTCAGTTCAGGAACCTGAGCCACAATGAACCGCACATGCCAC
AAAACGCCACGTTTCCCGATTCTTTCCACCAACCCAACAACGCTCCTTTCCCCTTATCTCCTAACAGCCC
CTATCCTCCTTCCCCTGCTAGCAGCACATATCCCAACTCCCCAGCAAGCTCTGGACCTGGAAGTCCATTT
CAACTCCCAGCTGACACCCCTCCCCCTGCCTATATGCCACCTGATGATCAGATGGCCCCAGATAATTCCC
AGCCTATGGATACAAGCAGTAACATGATTCCTCAGACCATGCCCAGCATATCCAGCAGAGATGTTCAGCC
TGTCGCCTATGAGGAGCCCAAACACTGGTGTT
nt 1127 - nt 1508 of NM_008541.1
Notes:The probe used in this study by Tremblay et al., 2001 [PMID:11566864] is indicated as "the proline-rich linker domain of Smad5 (Meersseman et al. 1997 [PMID:9076683] )" . Meersseman describe the probe: "proline rich 'variable' domain MusMLP antisense probe was made of a subcloned 378bp long EcoRI-TaqI fragment (of pMLP1.2 - a 1.2kb Madh5 cDNA)"
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:MF1
Age:8.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Tremblay et al., 2001 [PMID:11566864] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11566864] Tremblay KD, Dunn NR, Robertson EJ 2001 Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development (128):3609-21
 [ doi:10.1016/S0925-4773(96)00629-6] [ PMID:9076683] Meersseman G, Verschueren K, Nelles L, Blumenstock C, Kraft H, Wuytens G, Remacle J, Kozak CA, Tylzanowski P, Niehrs C, Huylebroeck D 1997 The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation. Mech Dev (61):127-40
Links:MGI:2155960 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE