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EMAGE:5584

Pcsk5 proprotein convertase subtilisin/kexin type 5 ( MGI:97515)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:5584
Scale bar is 200 microns. Anterior view.

Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo mesoderm
detected detected
The submitter describes expression in the lateral plate mesoderm.
embryo
detected detected
The submitter describes the expression as widespread.
extraembryonic component
detected detected
The submitter describes the expression as widespread.
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3064022
Entity Detected:Pcsk5, proprotein convertase subtilisin/kexin type 5 ( MGI:97515)
Sequence:sense strand is shown

>MGI:3064022
CCCACAGATGACTACGGTGCTGAAGATTATGCAGGTCCCTGTGACCCTGAATGCAGTGAGGTTGGATGTG
ACGGGCCAGGACCAGATCACTGCAGTGACTGCTTACACTACTACTACAAGCTGAAAAATAACACCAGAAT
CTGTGTCTCCAGCTGCCCTCCTGGCCACTACCATGCTGACAAGAAGAGATGCCGGAAGTGTGCCCCAAAC
TGCGAGTCCTGCTTTGGCAGCCATGGTAATCAGTGCCTCTCCTGTAAATATGGCTACTTCCTGAATGAAG
AAACTAGCAGCTGTGTTACTCAGTGCCCTGATGGATCATACGAGGATATCAAGAAAAATGTCTGTGGGAA
ATGCAGTGAGAACTGCAAGGCATGCATTGGATTTCACAACTGCACAGAGTGCAAGGGCGGGTTAAGTCTT
CAGGGATCCCGCTGTTCGGTCACCTGCGAGGATGGACAGTTCTTCAATGGTCACGACTGCCAGCCCTGCC
ATCGCTTCTGTGCTACTTGCTCTGGGGCCGGAGCAGATGGATGTATTAACTGCACCGAGGGGTATGTCAT
GGAAGAGGGAAGGTGTGTACAAAGTTGTAGTGTGAGCTACTACTT
Notes:Submitter information: H3061D03 is from NIA Mouse 15K cDNA Clone Set. The probe was made from the entire insert sequence of the clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE