Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5585

Pcsk5 proprotein convertase subtilisin/kexin type 5 ( MGI:97515)
TS14 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:5585
Scale bar is 200 microns. Lateral view.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5585Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5585_wholemount_moderate_3D_1.wlz
5585_wholemount_possible_3D_1.wlz
5585_wholemount_notDetected_3D_1.wlz
5585_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5585_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
The submitter describes regionally specific expression in the somites.
embryo
detected detected
regionalThe author describes regionally specific expression in various tissues.
unsegmented mesenchyme
detected detected
The submitter describes expression in the presomitic mesoderm.
Annotation Validation: Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3064022
Entity Detected:Pcsk5, proprotein convertase subtilisin/kexin type 5 ( MGI:97515)
Sequence:sense strand is shown

>MGI:3064022
CCCACAGATGACTACGGTGCTGAAGATTATGCAGGTCCCTGTGACCCTGAATGCAGTGAGGTTGGATGTG
ACGGGCCAGGACCAGATCACTGCAGTGACTGCTTACACTACTACTACAAGCTGAAAAATAACACCAGAAT
CTGTGTCTCCAGCTGCCCTCCTGGCCACTACCATGCTGACAAGAAGAGATGCCGGAAGTGTGCCCCAAAC
TGCGAGTCCTGCTTTGGCAGCCATGGTAATCAGTGCCTCTCCTGTAAATATGGCTACTTCCTGAATGAAG
AAACTAGCAGCTGTGTTACTCAGTGCCCTGATGGATCATACGAGGATATCAAGAAAAATGTCTGTGGGAA
ATGCAGTGAGAACTGCAAGGCATGCATTGGATTTCACAACTGCACAGAGTGCAAGGGCGGGTTAAGTCTT
CAGGGATCCCGCTGTTCGGTCACCTGCGAGGATGGACAGTTCTTCAATGGTCACGACTGCCAGCCCTGCC
ATCGCTTCTGTGCTACTTGCTCTGGGGCCGGAGCAGATGGATGTATTAACTGCACCGAGGGGTATGTCAT
GGAAGAGGGAAGGTGTGTACAAAGTTGTAGTGTGAGCTACTACTT
Notes:Submitter information: H3061D03 is from NIA Mouse 15K cDNA Clone Set. The probe was made from the entire insert sequence of the clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE