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EMAGE:569

Afp alpha fetoprotein ( MGI:87951)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:569
Figure 5A of Tremblay et al., 2001 [PMID:11566864] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:569Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
569_wholemount_strong_3D_1.wlz
569_wholemount_possible_3D_1.wlz
569_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:569_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
visceral extraembryonic endoderm
detected detected
regionalExpression is detected in the visceral endoderm overlying the embryonic region.
trophectoderm
not detected not detected
homogeneousExpression is absent from the extra-embryonic ectoderm.
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2678373
Entity Detected:Afp, alpha fetoprotein ( MGI:87951)
Notes:The probe used in this study by Tremblay et al., 2001 [PMID:11566864] is indicated as that used by Waldrip et al, 1998 [PMID:9529255] ie. a "300bp fragment of AFP".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:MF1
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Tremblay et al., 2001 [PMID:11566864] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11566864] Tremblay KD, Dunn NR, Robertson EJ 2001 Mouse embryos lacking Smad1 signals display defects in extra-embryonic tissues and germ cell formation. Development (128):3609-21
 [ doi:10.1016/S0092-8674(00)81407-5] [ PMID:9529255] Waldrip WR, Bikoff EK, Hoodless PA, Wrana JL, Robertson EJ 1998 Smad2 signaling in extraembryonic tissues determines anterior-posterior polarity of the early mouse embryo. Cell (92):797-808
Links:MGI:2155932 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI