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EMAGE:5690

Cdkn1c cyclin-dependent kinase inhibitor 1C (P57) ( MGI:104564)
TS14 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:5690

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5690Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5690_wholemount__moderate.wlz
5690_wholemount__weak.wlz
5690_wholemount__notDetected.wlz
5690_wholemount__strong.wlz
5690_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5690_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
regionalExpression is described in the cardiac mesoderm.
trunk somite
detected detected
Expression is described in the somites.
embryo
detected detected
Expression is deteced in 'various tissues'.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3067248
Entity Detected:Cdkn1c, cyclin-dependent kinase inhibitor 1C (P57) ( MGI:104564)
Sequence:sense strand is shown

>MGI:3067248
CCTTCGTTCTACATTTTCTAGTTTCTCTTTTTGTTTTGCACTGAGAGCGAGTAGAGATTAAACATTTTAC
ATAAATGATTTAAAGCTTTACACCTTGGGACCAGCGTACTCCTTGCACATGGTACAGAGTGTTCTCAATA
CATTGCACAGTTTTCAGATTTCCACAAAACCGTGGGCTGCTCTACGCAACCATCTCCGGTTCCTGCTACA
TGAACGAAAGGTCCCAGCCGAAGCCCAGAGTTCTTCCATCGTCCGCTGCCCAGATGCCCAGCAAGTTCTC
TCTGGCCGTTAGCCTCTAAACTAACTCATCTCAGACGTTTGCGCGGGGTCTGCTCCACCGCGCCCACCCC
AGGAGCCACGTTTGGAGAGGGACACCCTGGAGGGACGTCGTTCGACGCCTTGTTCTCCTGCGCAGTTCTC
TTGCGCTTGGCGA
Notes:The antisense RNA in situ probe was transcribed from the H3097D03 cDNA clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.0 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE