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EMAGE:5698

Agpat1 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha) ( MGI:1932075)
TS13 (e8.5 dpc)
in situ hybridisation

Data Images
EMAGE:5698

Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural tube
possible possible
Staining pattern is described as weak to no staining (yet with some evidence of expression in the neural tube).
embryo
possible possible
Staining pattern is described as weak to no staining.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:RZPDp981D10176D
Entity Detected:Agpat1, 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha) ( MGI:1932075)
Sequence:sense strand is shown

>RZPDp981D10176D
TTTTTACAACAGGCCGCAGAGAACCCCACGTCTCTCTTTCTCTTTATTTTCAGTTGCTTGCTGTTATCCA
GATAATAAAAACCAACCACACAAAACTGTACCCCACCCGTTCCTCCTTCCCCCCAGTCTACCTCCTGGGT
TTTAGGAATGGGTCTAGAGTGGGAGGCAGGGGTGGCCAACGCCACTGGGAGGAAATGAAAATTGGC
Notes:The antisense RNA in situ probe was transcribed from the sequence-verified RZPDp981D10176D/LIONp462E09365 cDNA clone.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:e8.5 dpc
Theiler Stage:TS13
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE