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EMAGE:5703

Efhd2 EF hand domain containing 2 ( MGI:106504)
TS13 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:5703

Expression pattern clarity: two stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindgut diverticulum
detected detected
Expression is described in the hindgut.
embryo
detected detected
regionalExpression is described in the endoderm.
outflow tract
detected detected
heart
detected detected
regionalExpression is described in the inflow tract and the outflow tract.
hindgut diverticulum endoderm
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:RZPDp981A08165D
Entity Detected:Efhd2, EF hand domain containing 2 ( MGI:106504)
Sequence:sense strand is shown

>RZPDp981A08165D
TTTCTTTTTTTGGGAGGTACTGAGAGGGGGAACCAAAAATAAAATGCAAACAGCCAAAWAGTWACGAGAA
TTTATATTCATCTGTATAAACATATCCGCTAAGGCAAACGCAAGCCAGGGCCACGGCAGCTGGGCTGGGC
TGGGCTTGGCTCGGCCCCTCCGATTCTCATAGGTTCTAAAGACCGAAAAAATAAAACCTTTGCAAGTCCC
ACAGGGTCCCGGGGACTCTGGGGACATTGTAKTAAAATAAAGAGTTATCGAGCTTCACAAGCAGTGACAC
GCTGAACATTGCATAACGGTGGCCTTGGTGGCCGTTCACTTAGACTGGGTGACGTCACAGTTTCACAACA
CAGCAACGGACACGCACATACACACACGTGAGCAAGGCACATGGAGCTATAGAGGAACGGCACGGGTTTC
ACTGGTGGCCTGGGGAGTYCCCATGCGAGTCT
Notes:The antisense RNA in situ probe was transcribed from sequence-verified RZPDp981A08165D/LIONp462B08100 cDNA clone.
Chemistry:DNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS13
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Owen J. Tamplin, Doris Kinzel, Brian J Cox, Christine E. Bell, Janet Rossant and Heiko Lickert.
Principal investigator:Heiko Lickert, Institute of Stem Cell Research, Helmholtz Zentrum M�nchen German Research Center for Environmental Health (GmbH) Ingolst�dter Landstra�e 1, Neuherberg, Germany D-85764
Submitted by:Owen J Tamplin, The Hospital for Sick Children Research Institute, Rossant Lab Room 13-305 Developmental and Stem Cell Biology Program Toronto Medical Discovery Tower 101 College Street, Toronto, Canada M5G 1L7
Experiment type:screen
References:[ doi:10.1186/1471-2164-9-511] [ PMID:18973680] Tamplin OJ, Kinzel D, Cox BJ, Bell CE, Rossant J, Lickert H 0 Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives. BMC Genomics (9):511
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE