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EMAGE:572

Furin furin (paired basic amino acid cleaving enzyme) ( MGI:97513)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:572 EMAGE:572 EMAGE:572 EMAGE:572 EMAGE:572
Figure 1F of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 1G of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 1H of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 1I of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Figure 1J of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.
EMAGE:572
Figure 1K of Roebroek et al., 1998 [PMID:9811571] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The embryo in F was sectioned to produce images G-K. Abbreviations used : aip, anterior intestinal port; cip, caudal intestinal portal; cp, notochordal plate; cv, cardinal veins; en, endocardium; fg, foregut; h, heart; hg, hindgut; lp, lateral plate; mc, prospective myocardium; nf, neural fold; np, neural plate; so, somite; vys, visceral yolk sac
Expression Pattern Description
Spatial Annotation:
EMAGE:572Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
572_wholemount_strong_3D_1.wlz
572_wholemount_moderate_3D_1.wlz
572_wholemount_possible_3D_1.wlz
572_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:572_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
allantois
detected detected
regionalHigh expression levels are seen in the allantois (1F)
heart
strong strong
gradedStrong expression is seen in the primordial heart, with particularly high levels in the inflow tract and anterior intestinal portal (1F).
gut
detected detected
regionalExpression is seen throughout the foregut endoderm (1G,H) Expression is seen in the hindgut and caudal intestinal portal (1K).
notochordal plate
strong strong
regionalFig1J
trunk mesenchyme
detected detected
regionalExpression is seen in lateral plate mesoderm of trunk
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309863
Entity Detected:Furin, furin (paired basic amino acid cleaving enzyme) ( MGI:97513)
Sequence:sense strand is shown

>MGI:1309863
GGATCCGGCTGGCGAATGGGTCCTAGAGATTGAAAATACCAGTGAAGCCAACAACTATGGGACGCTGACC
AAGTTCACTCTCGTTCTGTATGGCACAGCCCCCGAGGGGCTCTCTACACCTCCAGAAAGCAGCGGCTGCA
AGACCCTCACATCCAGCCAGGCCTGCGTGGTGTGTGAGGAAGGTTACTCTCTGCACCAGAAAAGCTGTGT
CCAGCACTGCCCACCAGGCTTCATCCCCCAAGTCCTTGATACACACTACAGCACTGAGAATGATGTGGAG
ATCATCCGTGCCAGCGTCTGTACCCCCTGCCACGCCTCATGTGCCACTTGCCAGGGCCCGGCCCCCACAG
ACTGCCTCAGCTGCCCCAGCCATGCCTCCCTGGACCCAGTGGAGCAGACCTGCTCCCGGCAAAGCCAGAG
CAGCCGGGAGTCTCGGCCTCAGCAGCAGCCTCCTGCACTGCGCCCTGAGGTGGAGATGGAGCCCCGGCTG
CAGGCTGGGTTGGCCTCTCACCTGCCCGAGGTCCTGGCCGGCCTCAGCTGCCTTATCATCGTGCTCATCT
TTGGCATCGTCTTCCTGTTCCTGCATCGGTGTTCGGGCTTCAGCTTCCGGGGAGTGAAAGTGTACACCAT
GGACCGCGGCCTCATCTCCTACAAGGGGCTGCCCCCTGAAGCCTGGCAGGAGGAGTGCCCATCGGACTCA
GAGGAGGACGAGGGCCGGGGCGAGAGGACCGCCTTCATCAAAGACCAGAGCGCCCTTTGAC
nt 2014 - nt 2774 of L26489.1
Notes:The probe used in this study by Roebroek et al., 1998 [PMID:9811571] is described as "nucleotides 2014-2774, corresponding to the 3' end of the Furin coding sequence (gb:L26489)".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Roebroek et al., 1998 [PMID:9811571] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9811571] Roebroek AJ, Umans L, Pauli IG, Robertson EJ, van Leuven F, Van de Ven WJ, Constam DB 1998 Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase Furin. Development (125):4863-76
Links:MGI:1329808 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI