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EMAGE:5721

Lmnb1 lamin B1 ( MGI:96795)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5721 EMAGE:5721 EMAGE:5721 EMAGE:5721 EMAGE:5721
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:5721 EMAGE:5721
Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + BM Purple. It has been imaged using Optical Projection Tomography. The visible channel depicting the signal has been false-coloured green and the fluorescence channel depicting autofluorescence has been false-coloured blue.
Expression Pattern Description
Spatial Annotation:
EMAGE:5721Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5721_wholemount__moderate.wlz
5721_wholemount__weak.wlz
5721_wholemount__notDetected.wlz
5721_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5721_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lmnb1_probeA
Entity Detected:Lmnb1, lamin B1 ( MGI:96795)
Sequence:sense strand is shown

>Lmnb1_probeA
GCGAATCTGATGGCCTTAATTTCCTTTTTGACACTGAAAGTTTTGTAAAAGAAATCCTATCCATACACGT
TGTAAGATGTGAATTATTGACACTGAGCTAACTGTACTGTTTGGAAAGGGGCCCTCAAGTTTTTGGCATT
TTTCTTTCCTTTTTGTATGTGTGTATGTAATTTTTTTTTTTTAAGTTCTTTTAAGGGGGGACAAGGAGGG
TAAGAAAACCACTGCGTGTCCGGGCATAATTGAAGCTTGCTCTCCCTAGATGGGTGGTCTGCTCTCGGTC
CTTCTCTGCTCTCTATAAAATGGTGCTGTGCGGGAGGGAGGGGGGAAGTTTTTCAATATATGAACTTTTG
TATGGAATTTTTTTGTAATAAGTGATCAAGGTTACAAATTTTTTTTTTAAATAGAAAAGAGAAGAAAAAC
GTTGTAAGAAGGCAATATTAATCTAGTCACCATGTACGCACTCTGGATGGAG
nt 56912421 - nt 56912892 of NC_000084.5
Notes:This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The antisense probe was transcribed using the T7 polymerase. Primers: T3 - 5' AATTAACCCTCACTAAAGGGCGAATCTGATGGCCTTAAT 3' T7 - 5' TAATACGACTCACTATAGGCTCCATCCAGAGTGCGTACA 3' Product length: 472bp
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Peter Branney, David R FitzPatrick
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Peter Branney, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE