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EMAGE:5732

Hhat hedgehog acyltransferase ( MGI:2444681)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5732
Figure 2P. [doi:10.1101/gad.1185804] Chen MH; Li YJ; Kawakami T; Xu SM; Chuang PT, "Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates." Genes Dev 2004; 18: 641-59. [PMID:15075292]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5732Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5732_wholemount__moderate.wlz
5732_wholemount__weak.wlz
5732_wholemount__possible.wlz
5732_wholemount__notDetected.wlz
5732_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5732_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Expression is widespread.
branchial arch
strong strong
embryo ectoderm
strong strong
Expression is stronger in the anterior ectoderm.
limb
strong strong
regionalExpression is strong in the distal part of the limb.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3052524
Entity Detected:Hhat, hedgehog acyltransferase ( MGI:2444681)
Notes:The Hhat (Skn) probe used in this study by Chen et al., 2004 [PMID:15075292] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Chen MH; Li YJ; Kawakami T; Xu SM; Chuang PT, 2004 [PMID:15075292] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.1185804] [ PMID:15075292] Chen MH, Li YJ, Kawakami T, Xu SM, Chuang PT 2004 Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates. Genes Dev (18):641-59
Links:MGI:3053341 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI