Type: | in situ hybridisation probe |
Identifier: | Crabp2_probeB |
Entity Detected: | Crabp2, cellular retinoic acid binding protein II ( MGI:88491) |
Sequence: | sense strand is shown
>Crabp2_probeB
TGACAGCAGATGACGTTGTGTGCACCAGGGTCTACGTCCGAGAGTGAGTGCCTACGGGTCCAAGAACTGC
CTGAGACGACTTCCGTGCCCGCTACAGGACACAAACCTCCCTCCCACGTCCATCTTACAAACTAGCTCTC
CCCTTACTCCTGAGGGTTACTGCTTCCTCCAAGGCCTTTTGTTCTTTGCCTTCTCTAAGCCAGAGAGGGG
CAGAAGCTCAGAACCCTCCCACCGCCATTTGCCCCTCCCAGGTCAGCAGTCCCAGCTCCACACCAGGGTC
CTTCCTGGAAGAGACTGTCTCTCTGGCCTCTACTCCTTATCCTTGTAGTCTGTGTGATT
|
| nt 87756913 - nt 87757251 of NC_000069.5 |
Notes: | This probe was transcribed from template produced by a PCR of whole genomic DNA using primers incorporating T3 and T7 promotors. The probes were transcribed using the T7 polymerase.
Primers:
T3 - 5' AATTAACCCTCACTAAAGGTGACAGCAGATGACGTTGTG 3'
T7 - 5' TAATACGACTCACTATAGGAATCACACAGACTACAAGGATAAGGA 3'
Product length: 339bp |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |