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EMAGE:5776

Hira histone cell cycle regulation defective homolog A (S. cerevisiae) ( MGI:99430)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5776
Figure 6E. Wilming LG; Snoeren CA; van Rijswijk A; Grosveld F; Meijers C, "The murine homologue of HIRA, a DiGeorge syndrome candidate gene, is expressed in embryonic structures affected in human CATCH22 patients." Hum Mol Genet 1997; 6: 247-58. [PMID:9063745]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: in the paper, Fig 6F shows a control embryo hybridised with a sense probe, which shows virtually no staining.
Expression Pattern Description
Spatial Annotation:
EMAGE:5776Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5776_wholemount__moderate.wlz
5776_wholemount__weak.wlz
5776_wholemount__possible.wlz
5776_wholemount__notDetected.wlz
5776_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5776_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Ubiquitously expressed.
1st branchial arch maxillary component mesenchyme
strong strong
regionalTranscripts are detected in the anterior part (including the maxillary component) of arch 1. Sections of gelatin embedded whole mount embryos reveal Hira transcripts in the mesenchyme of the pharyngeal arches (not shown).
2nd branchial arch mesenchyme
strong strong
regionalExpressed in the posterior region. Sections of gelatin embedded whole mount embryos reveal Hira transcripts in the mesenchyme of the pharyngeal arches (not shown).
fronto-nasal process
strong strong
limb
strong strong
head mesenchyme derived from neural crest
detected detected
Just posterior to arches 1 and 2 a circumscribed spot with a high level of Hira transcripts is present. This region corresponds well to the region where hindbrain neural crest cells reside before entering the developing gut and the third and fourth pharyngeal arch. In chicken embryos this region is known as the circumpharyngeal neural crest.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hira probeA
Entity Detected:Hira, histone cell cycle regulation defective homolog A (S. cerevisiae) ( MGI:99430)
Sequence:sense strand is shown

>Hira probeA
GCGCCTCAAAGCCTTGCACAGAACCAGTGGCAGCCACCAGTGCCAGGCCTACAGGCGAATCTGTCAGTAA
GGACAGTATGAATGCTACCTCTACTCCTGCTGCATCGTCACCCTCTGTGTTAACAACCCCATCCAAGATT
GAACCCATGAAAGCATTTGATTCCCGGTTCACAGAACGGTCCAAAGCCACACCAGGTGCTCCTTCCTTGA
CCAGTGTGATTCCAACGGCTGTTGAAAGGTTGAAAGAGCAGAACCTCGTCAAGGAGCTGAGGTCCCGGGA
ACTGGAGAGCAGCAGTGACAGCGATGAGAAGGTCCACCTAGCCAAGCCCTCTTCACTGTCCAAGCGCAAA
CTTGAGCTTGAGGTAGAGACGGTGGAAAAGAAGAAGAAAGGTCGCC
nt 1595 - nt 1990 of X99712.1
Notes:The Hira probe used in this study by Wilming et al., 1997 [PMID:9063745] was the "single stranded RNA probe mA, that only detects the 4.4 kb transcript". Editors Note: mA is a NarI-StyI fragment of the cDNA (see Figure 1 therein). X99712.1 is the mouse Hira cDNA sequence submitted by Wilming et al. The start and end co-ordinates of the probe relative to X99712.1 was deduced using this information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/N
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Wilming LG; Snoeren CA; van Rijswijk A; Grosveld F; Meijers C, 1997 [PMID:9063745] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/hmg/6.2.247] [ PMID:9063745] Wilming LG, Snoeren CA, van Rijswijk A, Grosveld F, Meijers C 1997 The murine homologue of HIRA, a DiGeorge syndrome candidate gene, is expressed in embryonic structures affected in human CATCH22 patients. Hum Mol Genet (6):247-58
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE