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EMAGE:5778

Hmgcr 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ( MGI:96159)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5778
Figure 6B. [doi:10.1210/me.2002-0436] Marijanovic Z; Laubner D; Moller G; Gege C; Husen B; Adamski J; Breitling R, "Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis." Mol Endocrinol 2003; 17: 1715-25. [PMID:12829805]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5778Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5778_wholemount__moderate.wlz
5778_wholemount__weak.wlz
5778_wholemount__possible.wlz
5778_wholemount__notDetected.wlz
5778_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5778_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
moderate moderate
Ubiquitous expression.
trigeminal v ganglion
strong strong
dorsal root ganglion
strong strong
forelimb bud
strong strong
regionalExpression is in the distal tip of the forelimb buds.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3663054
Entity Detected:Hmgcr, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ( MGI:96159)
Sequence:sense strand is shown

>MGI:3663054
TGTCAGAAGTCACATGGTTCACAACAGATCAAAGATAAATTTACAAGATCTTCAAGGAACGTGCACCAAG
AAGGCAGCTTGAGGATCCTGACACTGAACTGAAGCGCGGGCATTGGGTTCTCAAGGACTAACATGCAATC
TGTGAATTAAAAATCTCAATGCACTGTCTAGTGGAAGATGAATGGACATGATCAGTGACACCCCTGCTTG
GTTTCTGGCGCTTTCAGAGACGTCTGAGGTTCTTTGCACAGAGACTCCTCAGATGTGGGAACTCTGGTTC
TTTCCGTGCTGTGTTCTGGAGAGATCTCACGTGGATGGTACCGGTGCTCTGAGCACCACAGATGTGAGCT
ACAGTTCGTTTCTGAAAGCTACCACAAGCTGGAAACTGGTGATCAGTGTGGGGCTCACCTCTCCGTGGGT
TAAAAATGGTTTTAAATGACACTGTAGCTGACAG
nt 2732 - nt 3185 of NM_008255.2
Notes:The Hmgcr probe used in this study by Marijanovic et al., 2003 [PMID:12829805] was PCR-cloned from cDNA of NIH3T3 cells using CCTGTCAGAAGTCACATGGTTCAC and CTGTCAGCTACAGTGTCATTTAAAACC (see Table 2 therein).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C3HeB/FeJ
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Marijanovic Z; Laubner D; Moller G; Gege C; Husen B; Adamski J; Breitling R, 2003 [PMID:12829805] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1210/me.2002-0436] [ PMID:12829805] Marijanovic Z, Laubner D, Moller G, Gege C, Husen B, Adamski J, Breitling R 2003 Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis. Mol Endocrinol (17):1715-25
Links:MGI:3663117 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI