Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5780

Hnrnpd heterogeneous nuclear ribonucleoprotein D ( MGI:101947)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5780 EMAGE:5780
Fig1A. Gouble A; Morello D, "Synchronous and regulated expression of two AU-binding proteins, AUF1 and HuR, throughout murine development" Oncogene 2000; 19: 5377-84. [PMID:11103939] © Nature Publishing Group. Reproduced with permission from Oncogene. Fig1D. Gouble A; Morello D, "Synchronous and regulated expression of two AU-binding proteins, AUF1 and HuR, throughout murine development" Oncogene 2000; 19: 5377-84. [PMID:11103939] © Nature Publishing Group. Reproduced with permission from Oncogene.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: embryos stained with Hnrnpd (Auf1) sense probe, do not appear to show any staining (Fig1D). Image annotations: lb: limb bud, nt: neural tube and ba: branchial arches.
Expression Pattern Description
Spatial Annotation:
EMAGE:5780Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5780_wholemount__moderate.wlz
5780_wholemount__weak.wlz
5780_wholemount__possible.wlz
5780_wholemount__notDetected.wlz
5780_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5780_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
branchial arch
detected detected
neural tube
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hnrnpd probeA
Entity Detected:Hnrnpd, heterogeneous nuclear ribonucleoprotein D ( MGI:101947)
Sequence:sense strand is shown

>Hnrnpd probeA
GATCTGAAGGACTACTTTTCCAAATTTGGTGAAGTTGTAGACTGCACTCTGAAGTTAGATCCTATCACAG
GGCGATCAAGGGGTTTTGGCTTTGTGCTATTTAAAGAGTCGGAGAGTGTAGATAAGGTCATGGATCAGAA
AGAACATAAATTGAATGGGAAAGTCATTGATCCTAAAAGGGCCAAAGCCATGAAAACAAAAGAGCCTGTC
AAAAAAATTTTTGTTGGTGGCCTTTCTCCAGACACACCTGAAGAAAAAATAAGAGAGTACTTTGGTGGTT
TTGGTGAGGTTGAATCCATAGAGCTCCCTATGGACAACAAGACCAATAAGAGGCGTGGGTTCTGTTTTAT
TACCTTTAAGGAAGAGGAGCCAGTGAAGAAGATAATGGAAAAGAAATACCACAATGTTGGTCTTAGTAAA
TGTGAAATAAAAGTAGCCATGTCAAAGGAACAGTATCAGCAGCAGCAGCAGTGGGGAT
nt 648 - nt 1125 of NM_001077265.1
Notes:The Hnrnpd (Auf1) probe used in this study by Gouble & Morello, 2000 [PMID:11103939] was "a 478-nt-long BglII-XbaI fragment of AUF1 which hybridizes with the four AUF1 mRNAs". Editros note: the RefSeq sequences for the four transcript variants are: NM_001077265.1, NM_001077266.1, NM_007516.2 and NM_001077267.1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6 x CBA
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Gouble A & Morello D, 2000 [PMID:11103939] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/sj.onc.1203910] [ PMID:11103939] Gouble A, Morello D 2000 Synchronous and regulated expression of two AU-binding proteins, AUF1 and HuR, throughout murine development. Oncogene (19):5377-84
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE