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EMAGE:5781

Hoxa4 homeobox A4 ( MGI:96176)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5781
Figure 3A2. [doi:10.1046/j.1440-169x.2002.00623.x] Kawazoe Y; Sekimoto T; Araki M; Takagi K; Araki K; Yamamura K, "Region-specific gastrointestinal Hox code during murine embryonal gut development." Dev Growth Differ 2002; 44: 77-84. [PMID:11869294]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5781Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5781_wholemount__moderate.wlz
5781_wholemount__notDetected.wlz
5781_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5781_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalThis expression pattern is not described in detail by the authors, although they do state that the anterior expression boundary is generally consistent with that previously reported.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Hoxa4 probeA
Entity Detected:Hoxa4, homeobox A4 ( MGI:96176)
Notes:The Hoxa4 probe used in this study by Kawazoe et al., 2002 [PMID:11869294] was "provided by Professor K. Shimada (Osaka University, Japan)". Editors Note: in Figure 2 therein a cartoon shows that the probe is 750nt in length and covers the 3' UTR.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BDF1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kawazoe Y; Sekimoto T; Araki M; Takagi K; Araki K; Yamamura K, 2002 [PMID:11869294] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1046/j.1440-169x.2002.00623.x] [ PMID:11869294] Kawazoe Y, Sekimoto T, Araki M, Takagi K, Araki K, Yamamura K 2002 Region-specific gastrointestinal Hox code during murine embryonal gut development. Dev Growth Differ (44):77-84
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE