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EMAGE:5782

Hoxa5 homeobox A5 ( MGI:96177)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:5782 EMAGE:5782 EMAGE:5782
Figure 2E. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.03.008] Gene Expr Patterns 5: 792-800, Joksimovic M; Jeannotte L; Tuggle CK, "Dynamic expression of murine HOXA5 protein in the central nervous system." Copyright 2005. [PMID:15922675] Figure 2E1. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.03.008] Gene Expr Patterns 5: 792-800, Joksimovic M; Jeannotte L; Tuggle CK, "Dynamic expression of murine HOXA5 protein in the central nervous system." Copyright 2005. [PMID:15922675] Figure 2E2. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.03.008] Gene Expr Patterns 5: 792-800, Joksimovic M; Jeannotte L; Tuggle CK, "Dynamic expression of murine HOXA5 protein in the central nervous system." Copyright 2005. [PMID:15922675]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The approximate level of section E2 is shown by the black line in E. Asterisk and arrows indicate HOXA5 expression in the developing foregut and the anterior expression boundary in the neural tube, respectively. Black arrowheads show the anterior and posterior expression boundaries in somites. An open arrowhead depicts the protein localization in the developing scapula. lu, lung; o, otic vesicle; sc, spinal cord.
Expression Pattern Description
Spatial Annotation:
EMAGE:5782Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5782_wholemount__moderate.wlz
5782_wholemount__weak.wlz
5782_wholemount__notDetected.wlz
5782_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5782_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
foregut
detected detected
lung
detected detected
skeleton
detected detected
regionalExpressed in future scapula.
somite 06
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 07
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 14
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 13
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 12
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 11
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 10
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 09
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
somite 08
detected detected
Expression in somites was confined to a specific rostral-caudal region, approximately between somite 6-7 and somite 13-14
future spinal cord
detected detected
regional
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Hoxa5 antibodyA
Entity Detected:Hoxa5, homeobox A5 ( MGI:96177)
Notes:The anti-HOXA5 antibody used in this study by Joksimovic et al., 2005 [PMID:15922675] was produced thus: GST/HOXA5 exon 1 fusion protein was expressed in E. coli DH5alpha cells. The protein was affinity purified by incubation with Glutathione-Sepharose 4B, and eluted with glutathione. Immunization of two rabbits with the GST/HOXA5 exon I fusion protein was carried out. The crude sera was purified by three consecutive depletions using the following proteins/extracts coupled to CNBr-preactivated Sepharose. The sera was passed over columns that had covalently bound (a) whole bacterial extract containing GST/HOXB5 exon I fusion protein, (b) purified GST/HOXB5 exon I fusion protein or (c) purified GST protein, followed by affinity purification on a column with covalently bound purified GST/HOXA5 exon I fusion protein. In addition, the GST/HOXA5 exon I fusion protein bound to glutathione beads was digested by thrombin protease.
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Joksimovic M; Jeannotte L; Tuggle CK, 2005 [PMID:15922675] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.03.008] [ PMID:15922675] Joksimovic M, Jeannotte L, Tuggle CK 2005 Dynamic expression of murine HOXA5 protein in the central nervous system. Gene Expr Patterns (5):792-800
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE