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EMAGE:579

Hand2 heart and neural crest derivatives expressed transcript 2 ( MGI:103580)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:579
Figure 1O of Charite et al., 2000 [PMID:10804186] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Abbreviation used: flb, forelimb bud. White and black arrowheads point to posterior and proximal-anterior domains of expression, respectively, and green arrowhead to apical domain of intermediate expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:579Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
579_wholemount_strong_3D_1.wlz
579_wholemount_moderate_3D_1.wlz
579_wholemount_weak_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:579_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
regionalStrong expression detected in the posterior of the right forelimb. expression also detected in the proximal and anterior apical right forelimb bud.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1330181
Entity Detected:Hand2, heart and neural crest derivatives expressed transcript 2 ( MGI:103580)
Notes:The probe used in this study by Charite et al., 2000 [PMID:10804186] is indicated as that originally described in Srivastava et al., 1997 [PMID:9171826] ie. a "partial dHAND [Hand2] (nucleotide 292-644) cDNA". Probe ends are unspecified as numbering is not related to any particular Hand2 sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Charite et al., 2000 [PMID:10804186] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10804186] Charite J, McFadden DG, Olson EN 2000 The bHLH transcription factor dHAND controls Sonic hedgehog expression and establishment of the zone of polarizing activity during limb development. Development (127):2461-70
 [ doi:10.1038/ng0697-154] [ PMID:9171826] Srivastava D, Thomas T, Lin Q, Kirby ML, Brown D, Olson EN 1997 Regulation of cardiac mesodermal and neural crest development by the bHLH transcription factor, dHAND. Nat Genet (16):154-60
Links:MGI:1858450 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI