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EMAGE:5796

Igf1 insulin-like growth factor 1 ( MGI:96432)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:5796
Figure 1A. [doi:10.1152/ajpcell.00506.2006] Alfieri CM; Evans-Anderson HJ; Yutzey KE, "Developmental regulation of the mouse IGF-I exon 1 promoter region by calcineurin activation of NFAT in skeletal muscle." Am J Physiol Cell Physiol 2007; 292: C1887-94. [PMID:17229811]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5796Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5796_wholemount__moderate.wlz
5796_wholemount__weak.wlz
5796_wholemount__notDetected.wlz
5796_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5796_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
branchial arch
detected detected
embryo
detected detected
regionalExpressed in the developing face.
trunk somite
detected detected
Expressed in somites and somitic-derived musculature.
heart
possible possible
regional"Not predominant in cardiac muscle at this stage".
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Igf1 probeA
Entity Detected:Igf1, insulin-like growth factor 1 ( MGI:96432)
Sequence:sense strand is shown

>Igf1 probeA
GGCGGCTGCTTGTCCAACTTTTCTCTTTGGAAGGGGACTTTCGTGACTGAGCTGGGGTTTGAATTTTGGT
TTAAGGCTCTGCTCTTAGACTCACCGCGCGGCGCGGCAAGGCAAAGCCTCTTTAGGTGAATCGGCTGCTG
CTTGCCCCTGCTGCTGCTGTGCTGCCTCTGTCACTTGCCTCTGGCTCTGTCGCTCGCCCCTGCCGCTGCT
GCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCCGTTTGCCCCTGCCGCTGTCTATGTTCTGCCT
TCTCCTTTTGATCACTGGCCCTACTTCAAGTTTCCATTCTCAGCAAAATTATATCCTTCCAGACTTGTGT
TTTTTCAATTTGCAAGCACTTCTAAGCCGCTGTCACCGGCTCCTTGGAGGCAGTTGCCGGAGGGCTTAAT
TCATAAAAGATCCCAGTCAAAGAGTGCAGCGGGTTTTTTTTTTGTGCTGCATTCAAATGCTTCATCGCTA
TGAGTTTTTCTTAGGCAAGTAAGTGGCTTGGGACTTCGGGAGACAACCTTGTCAAGCACCTAATTGTGCC
GTCCTCGCGCTGCCAGCAAGCTTCAGCCACCTTACAGGAGTGAAGTCAGCGTTCATATACGCTGTGTGGT
CTGGCAGCTGGGATTGTGTGTCCCAAAGGGATTGTGGAATGTTACCTCGGCAGGCATGCATTCCACGTTT
GGAAAATCGGCTCCAAATGAACTTTCTTTCCCTGCTGGGTCTGCAGAACATTTCAGAAGCGGAGCTAGCA
ATACCCTTCAACTTTTTTCCTGTGCTTGCAGCAGGAGATGGCTGAAGGGAGCTACTGCATGCGTGGTGGT
CTTTGAAACATAGCTCAGGGAAGAGAGGTCAGCTGCCTAGAGCGATTGGTACACACAGGGCGCCTGCTGG
CTTTGTACTCTGAGGCTCAAGGTATTTCCCAGTGCCTGAGCCAGGGGACGATGGGGGAGGAAGGGAAGGA
nt 57 - nt 1036 of NM_184052.2
Notes:The Igf1 probe template used in this study by Alfieri et al., 2007 [PMID:17229811] was generated by RT-PCR from embryonic day (E)12.5 head using the following primers: forward: 5'-GGCGGCTGCTTGTCCAACTTTTCT-3'; reverse: 5'-TCCTTCCCTTCCTCCCCCATCGTC-3'. The amplified fragment was inserted into the pGEM T-vector (Promega) and verified by sequencing. It was linearized with NotI, and the digoxigenin-labeled antisense RNA probe was synthesized with T7 polymerase. This gives a "probe encompassing exons 3-6 (that are) present in both (the) exon 1- and exon 2-initiated transcripts".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Alfieri CM; Evans-Anderson HJ; Yutzey KE, 2007 [PMID:17229811] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1152/ajpcell.00506.2006] [ PMID:17229811] Alfieri CM, Evans-Anderson HJ, Yutzey KE 2007 Developmental regulation of the mouse IGF-I exon 1 promoter region by calcineurin activation of NFAT in skeletal muscle. Am J Physiol Cell Physiol (292):C1887-94
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE