Type: | in situ hybridisation probe |
Identifier: | Igf1 probeA |
Entity Detected: | Igf1, insulin-like growth factor 1 ( MGI:96432) |
Sequence: | sense strand is shown
>Igf1 probeA
GGCGGCTGCTTGTCCAACTTTTCTCTTTGGAAGGGGACTTTCGTGACTGAGCTGGGGTTTGAATTTTGGT
TTAAGGCTCTGCTCTTAGACTCACCGCGCGGCGCGGCAAGGCAAAGCCTCTTTAGGTGAATCGGCTGCTG
CTTGCCCCTGCTGCTGCTGTGCTGCCTCTGTCACTTGCCTCTGGCTCTGTCGCTCGCCCCTGCCGCTGCT
GCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCCGTTTGCCCCTGCCGCTGTCTATGTTCTGCCT
TCTCCTTTTGATCACTGGCCCTACTTCAAGTTTCCATTCTCAGCAAAATTATATCCTTCCAGACTTGTGT
TTTTTCAATTTGCAAGCACTTCTAAGCCGCTGTCACCGGCTCCTTGGAGGCAGTTGCCGGAGGGCTTAAT
TCATAAAAGATCCCAGTCAAAGAGTGCAGCGGGTTTTTTTTTTGTGCTGCATTCAAATGCTTCATCGCTA
TGAGTTTTTCTTAGGCAAGTAAGTGGCTTGGGACTTCGGGAGACAACCTTGTCAAGCACCTAATTGTGCC
GTCCTCGCGCTGCCAGCAAGCTTCAGCCACCTTACAGGAGTGAAGTCAGCGTTCATATACGCTGTGTGGT
CTGGCAGCTGGGATTGTGTGTCCCAAAGGGATTGTGGAATGTTACCTCGGCAGGCATGCATTCCACGTTT
GGAAAATCGGCTCCAAATGAACTTTCTTTCCCTGCTGGGTCTGCAGAACATTTCAGAAGCGGAGCTAGCA
ATACCCTTCAACTTTTTTCCTGTGCTTGCAGCAGGAGATGGCTGAAGGGAGCTACTGCATGCGTGGTGGT
CTTTGAAACATAGCTCAGGGAAGAGAGGTCAGCTGCCTAGAGCGATTGGTACACACAGGGCGCCTGCTGG
CTTTGTACTCTGAGGCTCAAGGTATTTCCCAGTGCCTGAGCCAGGGGACGATGGGGGAGGAAGGGAAGGA
|
| nt 57 - nt 1036 of NM_184052.2 |
Notes: | The Igf1 probe template used in this study by Alfieri et al., 2007 [PMID:17229811] was generated by RT-PCR from embryonic day (E)12.5 head using the following primers: forward: 5'-GGCGGCTGCTTGTCCAACTTTTCT-3'; reverse: 5'-TCCTTCCCTTCCTCCCCCATCGTC-3'. The amplified fragment was inserted into the pGEM T-vector (Promega) and verified by sequencing. It was linearized with NotI, and the digoxigenin-labeled antisense RNA probe was synthesized with T7 polymerase. This gives a "probe encompassing exons 3-6 (that are) present in both (the) exon 1- and exon 2-initiated transcripts". |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |