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EMAGE:5798

Igsf8 immunoglobulin superfamily, member 8 ( MGI:2154090)
TS17 (10.5 (early) dpc)
in situ hybridisation

Data Images
EMAGE:5798
Figure 3E. [doi:10.1016/S1044-7431(02)00021-0] Murdoch JN; Doudney K; Gerrelli D; Wortham N; Paternotte C; Stanier P; Copp AJ, "Genomic organization and embryonic expression of Igsf8, an immunoglobulin superfamily member implicated in development of the nervous system and organ epithelia." Mol Cell Neurosci 2003; 22: 62-74. [PMID:12595239]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5798Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5798_wholemount__moderate.wlz
5798_wholemount__possible.wlz
5798_wholemount__notDetected.wlz
5798_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5798_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
See arrows in Fig 3G.
gut
detected detected
regionalExpressed in a discrete region of the gut (see arrow in Fig 3H). Transverse sections reveal Igsf8 expression in the dorsal epithelium of the gut (white arrow, Fig3J).
neural tube
detected detected
regionalExpressed as a longitudinal stripe, which in dorsal view appears as two domains on either side of the midline. See Fig3F. Intense expression is apparent in the ventral one-third of the neural tube (black arrow, Fig3J,K) but with absence from the floor plate (white arrowhead, Fig3J) and from the region of differentiating motor neurons (black arrowhead, Fig3J)
3rd branchial arch ectoderm
detected detected
Transverse sections reveal Igsf8 expression in the mesodermal (arrow) and ectodermal (arrowhead) cell layers of the third pharyngeal arch (Fig3I)
3rd branchial arch mesenchyme
detected detected
Transverse sections reveal Igsf8 expression in the mesodermal (arrow) and ectodermal (arrowhead) cell layers of the third pharyngeal arch (Fig3I)
neural tube floor plate
not detected not detected
homogeneousAbsence of expression from the floor plate (white arrowhead, Fig3J)
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Igsf8 probeA
Entity Detected:Igsf8, immunoglobulin superfamily, member 8 ( MGI:2154090)
Sequence:sense strand is shown

>Igsf8 probeA
GCTGCAGGTATCTGCTGCCCCTCCAGGGCCCCGAGGGCGCCAGGCTGCCACCTCCCCCTCACGCCTCACA
GTGCACGAGGGGCAGGAGCTTGCGCTGGGCTGCCTGGCTCAGACCAAAACAAAGAAACACACACACCTGT
CGGTGTCCTTTGGGAGAGCCATTCCGGAGGCGCCAGTGGGGCGAGCCACTCTACAGGAAGTAGTGGGACT
GCGCTCCGACATGGCGGTGGAGGCTGGAGCGCCCTACGCTGAGAGGCTAGCTTCTGGGGAGCTTCGGCTG
AGCAAGGAAGGGACTGATCGGTACCGAATGGTGGTTGGGGGTGCCCAGGCTGGAGACTCGGGCACCTACC
ACTGTACGGCCGCTGAGTGGATTCAGGATCCTGACGGCTCCTGGGTCCAGGTCGCAGAGAAGAGGGCAGT
CCTGGCTCATGTTGATGTGCAGACTCTATCCAGCCAGCTGGCGGTGACCGTGGGACCTGGTGAACGTCGG
ATTGGCCCAGGCGAACCCTTAGAACTGCTGTGCAATGTGTCCGGTGCACTGCCCCCACCAGGCCGTCATG
CTGCGTACTCTGTGGGCTGGGAGATGGCCCCTGCAGGGGCTCCTGGACCCGGCCGCCTGGTGGCCCAGCT
AGACACGGAAGGTATAGGCAGCCTGGGCCCTGGCTATGAGGACCGGCACATTGCCATGGAGAAAGTAGCA
TCCAGAACCTACCGACTGCGGCTGGAGGCTGCCAGGCCCGCTGATGCAGGTACCTACCGCTGCCTCGCCA
AAG
nt 562 - nt 1334 of AF439263.1
Notes:The Igsf8 probe used in this study by Murdochet al., 2003 [PMID:12595239] "a 773-bp fragment of Igsf8, corresponding to nucleotides 562-1334 of GenBank sequence AF439263".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 (early) dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Murdoch JN; Doudney K; Gerrelli D; Wortham N; Paternotte C; Stanier P; Copp AJ, 2003 [PMID:12595239] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1044-7431(02)00021-0] [ PMID:12595239] Murdoch JN, Doudney K, Gerrelli D, Wortham N, Paternotte C, Stanier P, Copp AJ 2003 Genomic organization and embryonic expression of Igsf8, an immunoglobulin superfamily member implicated in development of the nervous system and organ epithelia. Mol Cell Neurosci (22):62-74
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE