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EMAGE:5802

Itga2b integrin alpha 2b ( MGI:96601)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:5802
Figure 4A. [doi:10.1182/blood-2002-06-1699] Mikkola HK; Fujiwara Y; Schlaeger TM; Traver D; Orkin SH, "Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo." Blood 2003; 101: 508-16. [PMID:12393529]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5802Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5802_wholemount__notDetected.wlz
5802_wholemount__weak.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5802_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
umbilical artery extraembryonic part
detected detected
At E9.5, CD41 expression was detected in the vitelline and umbilical vessels
vitelline vein embryonic part
detected detected
At E9.5, CD41 expression was detected in the vitelline and umbilical vessels
vitelline artery
detected detected
At E9.5, CD41 expression was detected in the vitelline and umbilical vessels
umbilical artery embryonic part
detected detected
At E9.5, CD41 expression was detected in the vitelline and umbilical vessels
umbilical vein embryonic part
detected detected
At E9.5, CD41 expression was detected in the vitelline and umbilical vessels
extraembryonic component
detected detected
Abundant expression of CD41 was detected in yolk sac blood islands.
cardiovascular system
detected detected
At E9.5, CD41 expression was detected in the vitelline and umbilical vessels; however, the endothelial cell network that stains with PECAM (not shown) in the embryo proper did not express detectable CD41.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Itga2b antibodyA
Entity Detected:Itga2b, integrin alpha 2b ( MGI:96601)
Notes:The anti-ITGA2B (CD41) antibody used in this study by Mikkola et al., 2003 [PMID:12393529] was obtained from BD Pharmingen. Editor's note: Editor's note: Pharmingen (BD Biosciences) sell three anti-mouse CD41 products - all are derived from one clone: MWReg30, which was produced from a rat injected with mouse platelets as immunogen. The three products differ as follows: Cat No. 553847 - purified; Cat No. 553848 - FITC congugated; Cat No. 558040 - PE (Phycoerythrin) conjugated. As the procedure used in this study did not utilise a fluorophore, it is assumed the non-fluorophore conjugated version (i.e. Cat No. 553847) was used.
Antibody Type:monoclonal
Raised In:rat
Supplier:BD Biosciences
Catalogue Number:553847
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Labelled with:horse radish peroxidase
General Information
Authors:Mikkola HK; Fujiwara Y; Schlaeger TM; Traver D; Orkin SH, 2003 [PMID:12393529] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1182/blood-2002-06-1699] [ PMID:12393529] Mikkola HK, Fujiwara Y, Schlaeger TM, Traver D, Orkin SH 2003 Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo. Blood (101):508-16
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE