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EMAGE:5813

Fn1 fibronectin 1 ( MGI:95566)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5813
Fig5A. from [doi:10.1111/j.1365-2133.2006.07399.x] Br J Dermatol 2006 155(3): 522-31, Yasuda M; Miyachi Y; Ishikawa O; Takahashi K, Spatial expressions of fibronectin and integrins by human and rodent dermal fibroblasts. Copyright 2006 [PMID:16911276]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5813Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5813_wholemount__moderate.wlz
5813_wholemount__weak.wlz
5813_wholemount__notDetected.wlz
5813_wholemount__strong.wlz
5813_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5813_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail
strong strong
regionalThe outgrowth edge of the tail strongly expresses FN mRNA.
embryo
detected detected
regionalIn the E10.5mouse embryo skin, hair follicles had apparently not yet emerged and the signals for FN were restricted to those body edges.
forelimb bud
strong strong
regionalExpression of FN in E10.5 was observed most strongly at the developing apex of limb buds.
hindlimb bud
strong strong
regionalExpression of FN in E10.5 was observed most strongly at the developing apex of limb buds.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fn1 probeA
Entity Detected:Fn1, fibronectin 1 ( MGI:95566)
Notes:The Fn1 (FN) probe used in this study by Yasuda et al., 2006 [PMID:16911276] is described as follows "The cDNA fragment covering the IIICS domain of the human FN gene was amplified by PCR and subcloned into a pBS/KS+ (Strategene, La Jolla, CA, U.S.A.) plasmid vector. Labelled cRNA probes were synthesized by in vitro transcription with digoxigenin-labelled UTP (Roche, Penzberg, Germany)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yasuda M; Miyachi Y; Ishikawa O; Takahashi K, 2006 [PMID:16911276] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1111/j.1365-2133.2006.07399.x] [ PMID:16911276] Yasuda M, Miyachi Y, Ishikawa O, Takahashi K 2006 Spatial expressions of fibronectin and integrins by human and rodent dermal fibroblasts. Br J Dermatol (155):522-31
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE