Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5814

Folr1 folate receptor 1 (adult) ( MGI:95568)
TS14 (15-16 Somite no.)
in situ hybridisation

Data Images
EMAGE:5814 EMAGE:5814
Fig3G. Copyright: This image is from [doi:10.1002/dvdy.10203] Saitsu H; Ishibashi M; Nakano H; Shiota K, "Spatial and temporal expression of folate-binding protein 1 (Fbp1) is closely associated with anterior neural tube closure in mice." Dev Dyn 2003 Jan;226(1):112-7. Reprinted with permission of Wiley-Liss Inc. [PMID:12508232] . Fig3H. Copyright: This image is from [doi:10.1002/dvdy.10203] Saitsu H; Ishibashi M; Nakano H; Shiota K, "Spatial and temporal expression of folate-binding protein 1 (Fbp1) is closely associated with anterior neural tube closure in mice." Dev Dyn 2003 Jan;226(1):112-7. Reprinted with permission of Wiley-Liss Inc. [PMID:12508232] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The broken lines indicate the boundaries between the closures. The double-ended arrow indicates the r (rhombomere) 1 segment. O, otic placode; 1, closure region 1; 2, closure region 2; 3, closure region 3; 4, closure region 4; PN - posterior neuropore. Fig3g is a lateral view, Fig3h is a dorsal view.
Expression Pattern Description
Spatial Annotation:
EMAGE:5814Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5814_wholemount__moderate.wlz
5814_wholemount__weak.wlz
5814_wholemount__notDetected.wlz
5814_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5814_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
caudal neuropore
not detected not detected
homogeneousFbp1 expression was not detected in the posterior neuropore.
rhombomere 01
detected detected
regionalFbp1 expression was localized to the dorsal regions of the neural tube between the mid-prosencephalon and the r1.
future midbrain
detected detected
regionalFbp1 expression was localized to the dorsal regions of the neural tube between the mid-prosencephalon and the r1.
prosencephalon
detected detected
regionalFbp1 expression was localized to the dorsal regions of the neural tube between the mid-prosencephalon and the r1.
rostral neuropore
not detected not detected
homogeneousFbp1 expression was not detected in the closure 3 region.
rhombomere 02
detected detected
regionalThe dorsal part of the presumptive r2 region showed weak expression of Fbp1 at the 8-somite stage, and it became more intense by the 16-somite stage (i.e. the stage shown in this EMAGE entry).
future hindbrain
weak weak
regionalThe remaining part of the closure 4 region (i.e. not r1 or r2), showed weak expression of Fbp1 before closure 4 was completed.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Folr1 probeA
Entity Detected:Folr1, folate receptor 1 (adult) ( MGI:95568)
Notes:The Folr1 (Fbp1) probe used in this study by Saitsu et al., 2003 [PMID:12508232] is one of two probes used that are described as follows: "We purchased two IMAGE cDNA clones (Lennnon et al., 1996 [PMID:8617505] ) from Research Genetics (Huntsville, AL). The insertions of the clones (ID: 3592216, 1514896) corresponded to nucleotide positions 13-669 and 280-677, respectively, in the mouse Fbp1 cDNA (Brigle et al., 1991 [PMID:1894617] ). These two different antisense probes demonstrated the identical staining patterns." Editors note: The sequences of the probes can be defined as follows, nucleotides 13-669 of M64782.1 and nucleotides 280-677 of M64782.1. However, it is not indicated in the paper which of the probes was used for this particular assay.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:15-16 Somite no.
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Saitsu H; Ishibashi M; Nakano H; Shiota K, 2003 [PMID:12508232] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.10203] [ PMID:12508232] Saitsu H, Ishibashi M, Nakano H, Shiota K 2003 Spatial and temporal expression of folate-binding protein 1 (Fbp1) is closely associated with anterior neural tube closure in mice. Dev Dyn (226):112-7
 [ doi:10.1006/geno.1996.0177] [ PMID:8617505] Lennon G, Auffray C, Polymeropoulos M, Soares MB 1996 The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics (33):151-2
 [ PMID:1894617] Brigle KE, Westin EH, Houghton MT, Goldman ID 1991 Characterization of two cDNAs encoding folate-binding proteins from L1210 murine leukemia cells. Increased expression associated with a genomic rearrangement. J Biol Chem (266):17243-9
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE