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EMAGE:5830

Frs2 fibroblast growth factor receptor substrate 2 ( MGI:1100860)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5830 EMAGE:5830 EMAGE:5830
Fig1M. Copyright: This image is from [doi:10.1073/pnas.0407577101] Gotoh N; Ito M; Yamamoto S; Yoshino I; Song N; Wang Y; Lax I; Schlessinger J; Shibuya M; Lang RA, Proc Natl Acad Sci U S A 2004 Dec 7;101(49):17144-9. [PMID:15569927] Copyright 2004 National Academy of Sciences, U.S.A. Fig1N. Copyright: This image is from [doi:10.1073/pnas.0407577101] Gotoh N; Ito M; Yamamoto S; Yoshino I; Song N; Wang Y; Lax I; Schlessinger J; Shibuya M; Lang RA, Proc Natl Acad Sci U S A 2004 Dec 7;101(49):17144-9. [PMID:15569927] Copyright 2004 National Academy of Sciences, U.S.A. Fig1O. Copyright: This image is from [doi:10.1073/pnas.0407577101] Gotoh N; Ito M; Yamamoto S; Yoshino I; Song N; Wang Y; Lax I; Schlessinger J; Shibuya M; Lang RA, Proc Natl Acad Sci U S A 2004 Dec 7;101(49):17144-9. [PMID:15569927] Copyright 2004 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
In situ hybridisations using Frs2alpha antisense (M) and control sense (N) probes on wild-type E9.5 embryos. (O) Cryosection of the eye region of the embryo shown in (M). Image annotations: ov, optic vesicle; br, branchial arch; op, otic pit; lb, limb bud; and lp, lens placode.
Expression Pattern Description
Spatial Annotation:
EMAGE:5830Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5830_wholemount__moderate.wlz
5830_wholemount__weak.wlz
5830_wholemount__strong.wlz
5830_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5830_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Frs2-alpha is expressed ubiquitously.
future forebrain
detected detected
Expressed "ubiquitously although mainly in the forebrain, eye primordia, first and second branchial arches, otic vesicle, and limb bud".
eye
detected detected
1st branchial arch
detected detected
Expressed "ubiquitously although mainly in the forebrain, eye primordia, first and second branchial arches, otic vesicle, and limb bud".
2nd branchial arch
detected detected
Expressed "ubiquitously although mainly in the forebrain, eye primordia, first and second branchial arches, otic vesicle, and limb bud".
limb
detected detected
Expressed "ubiquitously although mainly in the forebrain, eye primordia, first and second branchial arches, otic vesicle, and limb bud".
ear
detected detected
Expressed "ubiquitously although mainly in the forebrain, eye primordia, first and second branchial arches, otic vesicle, and limb bud".
optic vesicle
strong strong
regionalA horizontal section of a labeled embryo at the level of the eye primordium showed high Frs2alpha expression in the distal part of the optic vesicle that is the presumptive retina (Fig. 1O).
lens placode
strong strong
A horizontal section of a labeled embryo at the level of the eye primordium showed high Frs2alpha expression in the surface ectoderm, including the presumptive lens (Fig. 1O).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Frs2 probeA
Entity Detected:Frs2, fibroblast growth factor receptor substrate 2 ( MGI:1100860)
Notes:The Frs2 (Frs2-alpha) probe used in this study by Gotoh et al., 2004 [PMID:15569927] is indicated as that previously described by Gotoh et al., 2004 [PMID:15094036] i.e: "For whole-mount RNA in situ hybridization experiments, the mouse Frs2-alpha cDNA fragment linearized with HpaI was used as a specific probe." Editors Note: Gotoh et al., 2004 [PMID:15094036] do not reveal the identity of the Frs2-alpha clone used.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Gotoh N; Ito M; Yamamoto S; Yoshino I; Song N; Wang Y; Lax I; Schlessinger J; Shibuya M; Lang RA, 2004 [PMID:15569927] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0407577101] [ PMID:15569927] Gotoh N, Ito M, Yamamoto S, Yoshino I, Song N, Wang Y, Lax I, Schlessinger J, Shibuya M, Lang RA 2004 Tyrosine phosphorylation sites on FRS2alpha responsible for Shp2 recruitment are critical for induction of lens and retina. Proc Natl Acad Sci U S A (101):17144-9
 [ doi:10.1016/S0014-5793(04)00287-X] [ PMID:15094036] Gotoh N, Laks S, Nakashima M, Lax I, Schlessinger J 2004 FRS2 family docking proteins with overlapping roles in activation of MAP kinase have distinct spatial-temporal patterns of expression of their transcripts. FEBS Lett (564):14-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE