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EMAGE:5834

Frzb frizzled-related protein ( MGI:892032)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5834
Fig3B. Copyright: This image is from [doi:10.1002/(SICI)1097-0177(199807)212:3<364::AID-AJA4>3.0.CO;2-F] Hoang BH; Thomas JT; Abdul-Karim FW; Correia KM; Conlon RA; Luyten FP; Ballock RT, Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members. Dev Dyn 1998 Jul;212(3):364-72. Reprinted with permission of Wiley-Liss Inc. [PMID:9671940] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Arrow indicates the thickened oral epithelium. E, oral epithelium; EM, ectomesenchyme; FL, forelimb; Ha, hyoid arch; HL, hindlimb; ma, mandible; Md, mandibular division of the first branchial arch; Mx, maxillary division of the first branchial arch.
Expression Pattern Description
Spatial Annotation:
EMAGE:5834Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5834_wholemount__moderate.wlz
5834_wholemount__notDetected.wlz
5834_wholemount__strong.wlz
5834_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5834_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
strong strong
regionalIntense staining is observed in the ventral facial mesenchyme adjacent to the maxillary arch.
2nd branchial arch mesenchyme
detected detected
Staining extends into the mesenchyme of the hyoid or second branchial arch.
1st branchial arch mandibular component mesenchyme
detected detected
Staining is observed throughout the mesenchyme of the first arch.
1st branchial arch maxillary component mesenchyme
detected detected
Staining is observed throughout the mesenchyme of the first arch.
forelimb bud
detected detected
By 10.5 dpc, mFrzb-1 begins to lose its strictly ventral expression pattern in the forelimb.
hindlimb bud
detected detected
regionalAt 10.5 dpc, mFrzb-1 remains ventral in the hindlimb.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Frzb probeA
Entity Detected:Frzb, frizzled-related protein ( MGI:892032)
Notes:The Frzb (mFrzb-1) probe used in this study by Hoang et al., 1998 [PMID:9671940] is described as follows: "mFrzb-1 was linearized with NotI and transcribed with T7 RNA polymerase for the antisense probe." Editors Note: several Frzb clones are described by Hoang et al., and it is not specified which one is referred to above.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Hoang BH; Thomas JT; Abdul-Karim FW; Correia KM; Conlon RA; Luyten FP; Ballock RT, 1998 [PMID:9671940] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/(SICI)1097-0177(199807)212:3<364::AID-AJA4>3.0.CO;2-F] [ PMID:9671940] Hoang BH, Thomas JT, Abdul-Karim FW, Correia KM, Conlon RA, Luyten FP, Ballock RT 1998 Expression pattern of two Frizzled-related genes, Frzb-1 and Sfrp-1, during mouse embryogenesis suggests a role for modulating action of Wnt family members. Dev Dyn (212):364-72
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE