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EMAGE:5843

Fzd8 frizzled homolog 8 (Drosophila) ( MGI:108460)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5843
Fig2H. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2004.02.001] Gene Expr Patterns 4(5): 569-72, Lu CC; Robertson EJ; Brennan J, The mouse frizzled 8 receptor is expressed in anterior organizer tissues. Copyright 2004. [PMID:15261835]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: FB, Forebrain; S, Somite; PSM, Presomitic mesoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:5843Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5843_wholemount__moderate.wlz
5843_wholemount__weak.wlz
5843_wholemount__notDetected.wlz
5843_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5843_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
prosencephalon
detected detected
At somite stages Fzd8 expression is seen in the future forebrain region.
trunk somite
detected detected
At somite stages Fzd8 expression is seen in the segmenting somites.
unsegmented mesenchyme
strong strong
regionalHigh levels are seen in two stripes in the pre-somitic mesoderm, which is about to segment.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fzd8 probeC
Entity Detected:Fzd8, frizzled homolog 8 (Drosophila) ( MGI:108460)
Sequence:sense strand is shown

>Fzd8 probeC
ATTCGGGACGAGGTCGCTGTTCCGAATCCGTTCAGTCATCAAGCAGCAAGGAGGCCCAACTAAGACACAC
AAGCTAGAAAAACTCATGATCCGCTTGTGGCCTCTTCACCGTGCTCTACACGGTGCCCGCTGCCGTCGTT
GTCGCCTGCCTTTTCTATGAGCAGCACAACCGACCGCGCTGGGAGGCCACGCACAACTGCCCATGCCTTC
GGGACCTGCAACCGGACCAGGCTCGCAGGCCCGATTACGCGGTCTTCATGCTCAAGTACTTCATGTGCCT
AGT
Notes:The Fzd8 (mfz8) probe used in this study by Lu et al., 2004 [PMID:15261835] is described as follows: "An approximately 800 bp EST corresponding to the mfz8 gene isolated from a 13.5-14.5 dpc mouse embryo cDNA library (Genbank Accession No. W56988) was used to generate an RNA in situ hybridization probe".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Lu CC; Robertson EJ; Brennan J, 2004 [PMID:15261835] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2004.02.001] [ PMID:15261835] Lu CC, Robertson EJ, Brennan J 2004 The mouse frizzled 8 receptor is expressed in anterior organizer tissues. Gene Expr Patterns (4):569-72
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE