Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:5850

Gap43 growth associated protein 43 ( MGI:95639)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:5850 EMAGE:5850
Fig2E. Copyright: Reprinted with permission from the Society for Neuroscience from [doi:10.1523/JNEUROSCI.3455-05.2005] J Neurosci 25(44): 10119-30. Dillon AK; Fujita SC; Matise MP; Jarjour AA; Kennedy TE; Kollmus H; Arnold HH; Weiner JA; Sanes JR; Kaprielian Z. Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord. Copyright 2005. [PMID:16267219] Fig2F. Copyright: Reprinted with permission from the Society for Neuroscience from [doi:10.1523/JNEUROSCI.3455-05.2005] J Neurosci 25(44): 10119-30. Dillon AK; Fujita SC; Matise MP; Jarjour AA; Kennedy TE; Kollmus H; Arnold HH; Weiner JA; Sanes JR; Kaprielian Z. Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord. Copyright 2005. [PMID:16267219]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Anti-GAP43 (E, F) labels the SAN (i.e. spinal accessory nerve, arrow) and a significant number of axons/nerves in a whole E10.5 mouse embryo.
Expression Pattern Description
Spatial Annotation:
EMAGE:5850Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5850_wholemount__moderate.wlz
5850_wholemount__weak.wlz
5850_wholemount__notDetected.wlz
5850_wholemount__strong.wlz
5850_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5850_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
detected detected
Gap43 labels the dorsal root ganglia.
cranial ganglion
detected detected
Gap43 labels the cranial ganglia.
peripheral nervous system
detected detected
regionalGap43 labels the dorsal root ganglia, ventral roots, and cranial ganglia.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Gap43 antibodyA
Entity Detected:Gap43, growth associated protein 43 ( MGI:95639)
Notes:The anti-Gap43 antibody used in this study by Dillon et al., 2005 [PMID:16267219] is described as "mAb 347 (anti-GAP43; mouse IgG; Chemicon)." Editors note: When this EMAGE entry was made, Chemicon had been incorporated into Millipore, where the antibody can still be found (MAB347). The data sheet for the antibody refers to clone 9-1E12. The immunogen used was GAP-43 purified from rat brain. This antiserum recognises GAP-43 regardless of its phosphorylation state.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Millipore
Catalogue Number:MAB347
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:biotinylated anti-mouse
Tertiary Antibody:avidin biotinylated enzyme complex (ABC - Vector labs)
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Dillon AK; Fujita SC; Matise MP; Jarjour AA; Kennedy TE; Kollmus H; Arnold HH; Weiner JA; Sanes JR; Kaprielian Z, 2005 [PMID:16267219] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1523/JNEUROSCI.3455-05.2005] [ PMID:16267219] Dillon AK, Fujita SC, Matise MP, Jarjour AA, Kennedy TE, Kollmus H, Arnold HH, Weiner JA, Sanes JR, Kaprielian Z 2005 Molecular control of spinal accessory motor neuron/axon development in the mouse spinal cord. J Neurosci (25):10119-30
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE