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EMAGE:5853

Gcg glucagon ( MGI:95674)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5853
Fig6A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.ydbio.2004.10.012] Dev Biol 278(2): 484-95, Lee CS; Sund NJ; Behr R; Herrera PL; Kaestner KH, Foxa2 is required for the differentiation of pancreatic alpha-cells. Copyright 2004 [PMID:15680365]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: * - Pancreatic primordia.
Expression Pattern Description
Spatial Annotation:
EMAGE:5853Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5853_wholemount__possible.wlz
5853_wholemount__notDetected.wlz
5853_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5853_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
detected detected
Expressed in the pancreatic primordium
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gcg probeA
Entity Detected:Gcg, glucagon ( MGI:95674)
Notes:The Gcg (glucagon) probe used in this study by Lee et al., 2005 [PMID:15680365] is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Lee CS; Sund NJ; Behr R; Herrera PL; Kaestner KH, 2005 [PMID:15680365] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ydbio.2004.10.012] [ PMID:15680365] Lee CS, Sund NJ, Behr R, Herrera PL, Kaestner KH 2005 Foxa2 is required for the differentiation of pancreatic alpha-cells. Dev Biol (278):484-95
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE