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EMAGE:5856

Gfap glial fibrillary acidic protein ( MGI:95697)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:5856
Figure3B(wt) from [doi:10.1038/85855] Nat Genet 27(3):293-8. Guris DL; Fantes J; Tara D; Druker BJ; Imamoto A. Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. 2001. [PMID:11242111] © Nature Publishing Group. Reproduced with permission from Nat Genet.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ov, otic vesicle; V, Vth ganglion; VII, VIIth ganglion; VIII, VIIIth ganglion; IX, proximal IXth ganglion; IXd, distal IXth ganglion; X, proximal Xth ganglion; Xd, distal Xth ganglion; XI, XIth ganglion.
Expression Pattern Description
Spatial Annotation:
EMAGE:5856Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5856_wholemount__moderate.wlz
5856_wholemount__weak.wlz
5856_wholemount__notDetected.wlz
5856_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5856_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
acoustic viii ganglion
detected detected
facial vii ganglion
detected detected
glossopharyngeal ix ganglion
detected detected
trigeminal v ganglion
detected detected
vagus x ganglion
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Gfap antibodyA
Entity Detected:Gfap, glial fibrillary acidic protein ( MGI:95697)
Antigen:sense strand is shown

>Gfap antibodyA
GEVIKESKQEHKDVM
aa 418 - aa 432 of NP_002046.1
Notes:The anti-Gfap antibody used in this study by Guris et al., 2001 [PMID:11242111] is indicated as being sourced from Transduction Laboratories. Editors Note: Transduction Laboratories have been incorporated into BD Biosciences, who sell two Transduction Laboratories anti-GFAP products (610565 and 610566), both of which are from the one mouse clone (52), and differ only in the aliquot size. Information in the technical data sheet state that Clone 52 was produced by immunisation of a mouse with human GFAP (aa 418-432).
Antibody Type:monoclonal
Raised In:mouse
Supplier:BD Transduction Laboratories
Catalogue Number:either: 610565 or 610566
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol/DMSO 4:1
Secondary Antibody:anti-mouse IgG
Labelled with:peroxidase
Visualisation method:4-chloro-1-naphthol
General Information
Authors:Guris DL; Fantes J; Tara D; Druker BJ; Imamoto A, 2001 [PMID:11242111] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/85855] [ PMID:11242111] Guris DL, Fantes J, Tara D, Druker BJ, Imamoto A 2001 Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy neurocristopathies of DiGeorge syndrome. Nat Genet (27):293-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE