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EMAGE:5858

Ghitm growth hormone inducible transmembrane protein ( MGI:1913342)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5858
Fig1A(left). [doi:10.1016/j.bbrc.2005.12.141] Reprinted from Biochem Biophys Res Comm, vol 341, Yoshida T; Nagata S; Kataoka H, Ghitm is an ortholog of the Bombyx mori prothoracic gland-derived receptor (Pgdr) that is ubiquitously expressed in mammalian cells and requires an N-terminal signal sequence for expression, pp 13-18, Copyright (2005) with permission from Elsevier. [PMID:16412389]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5858Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5858_wholemount__moderate.wlz
5858_wholemount__notDetected.wlz
5858_wholemount__strong.wlz
5858_wholemount__possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5858_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
detected detected
regionalExpression was detected in a nerve cord projecting from the prosencephalon to the rhombencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3664727
Entity Detected:Ghitm, growth hormone inducible transmembrane protein ( MGI:1913342)
Sequence:sense strand is shown

>MGI:3664727
CTCCTGAGAGCCGCATGGTACACCGCTGGTATTGTGGGAGGCCTCTCTACTGTGGCCATGTGTGCGCCTA
GTGAGAAGTTTCTGAACATGGGAGCACCCCTGGGAGTGGGCCTGGGTCTTGTCTTTGCGTCTTCTCTGGG
GTCTATGTTTCTTCCCCCTACCTCTGTGGCTGGTGCCACTCTGTACTCAGTGGCAATGTATGGTGGATTA
GTTCTTTTCAGCATGTTCCTTCTGTATGATACTCAGAAAGTAATCAAACGTGCAGAAATAACACCCATGT
ATGGAGCTCAAAAGTATGATCCCATCAATTCGATGTTGACAATCTACATGGATACATTAAATATATTTAT
GCGAGTTGCAACTATGCTAGCAACTGGAAGCAACAGAAAGAAAT
nt 757 - nt 1150 of NM_078478.4
Notes:The region covered by the Ghitm probe used in this study by Yoshida et al., 2006 [PMID:16412389] is denoted by the authors in Figure2B with reference to the amino acid sequence of Ghitm - i.e. aa 215-346 of NP_510963.1. Editors Note: The probe region corresponds to nt 757-1150 of NM_078478.4.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BALB/c
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yoshida T; Nagata S; Kataoka H, 2006 [PMID:16412389] , Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.bbrc.2005.12.141] [ PMID:16412389] Yoshida T, Nagata S, Kataoka H 2006 Ghitm is an ortholog of the Bombyx mori prothoracic gland-derived receptor (Pgdr) that is ubiquitously expressed in mammalian cells and requires an N-terminal signal sequence for expression. Biochem Biophys Res Commun (341):13-8
Links:MGI:3664732 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI