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EMAGE:5860

Gja4 gap junction protein, alpha 4 ( MGI:95715)
TS15 (9.2 dpc)
in situ hybridisation

Data Images
EMAGE:5860
Figure 1D from [doi:10.1016/j.yjmcc.2005.02.021] J Mol Cell Cardiol 38(5):787-98. Dupays L; Jarry-Guichard T; Mazurais D; Calmels T; Izumo S; Gros D; Theveniau-Ruissy M. Dysregulation of connexins and inactivation of NFATc1 in the cardiovascular system of Nkx2-5 null mutants. Copyright 2005. [PMID:15850572]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5860Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5860_wholemount__moderate.wlz
5860_wholemount__weak.wlz
5860_wholemount__notDetected.wlz
5860_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5860_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
cardiovascular system
detected detected
regionalCx37 transcript was observed in the heart and the vessels of the embryos.
heart
detected detected
The Cx37 gene transcript, which is synthesized in the vascular and endocardial endothelial cells, but not in cardiomyocytes was detected in the heart.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gja4 probeA
Entity Detected:Gja4, gap junction protein, alpha 4 ( MGI:95715)
Notes:The Gja4 (Cx37) probe used in this study by Dupays et al., 2005 [PMID:15850572] is of unreported sequence. The in situ technique was carried out as described previously by Delorme et al., 1997 [PMID:9285645] .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.2 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dupays L; Jarry-Guichard T; Mazurais D; Calmels T; Izumo S; Gros D; Theveniau-Ruissy M, 2005 [PMID:15850572] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.yjmcc.2005.02.021] [ PMID:15850572] Dupays L, Jarry-Guichard T, Mazurais D, Calmels T, Izumo S, Gros D, Theveniau-Ruissy M 2005 Dysregulation of connexins and inactivation of NFATc1 in the cardiovascular system of Nkx2-5 null mutants. J Mol Cell Cardiol (2005):787-98
 [ PMID:9285645] Delorme B, Dahl E, Jarry-Guichard T, Briand JP, Willecke K, Gros D, Theveniau-Ruissy M 1997 Expression pattern of connexin gene products at the early developmental stages of the mouse cardiovascular system. Circ Res (1997):423-37
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE