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EMAGE:5861

Gjc1 gap junction protein, gamma 1 ( MGI:95718)
TS16 (10 dpc)
in situ hybridisation

Data Images
EMAGE:5861
Figure 2E from Circ Res 84(12):1365-79. Alcolea S; Theveniau-Ruissy M; Jarry-Guichard T; Marics I; Tzouanacou E; Chauvin JP; Briand JP; Moorman AF; Lamers WH; Gros DB. Downregulation of connexin 45 gene products during mouse heart development. Copyright 1999. [PMID:10381888]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5861Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5861_wholemount__moderate.wlz
5861_wholemount__weak.wlz
5861_wholemount__notDetected.wlz
5861_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5861_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
The transcript was detected in all of the cardiac compartments, including the inflow tract.
atrioventricular canal
detected detected
The most remarkable labelings were those of the atrioventricular canal and the outflow tract.
outflow tract
detected detected
The most remarkable labelings were those of the atrioventricular canal and the outflow tract.
future forebrain
strong strong
Strong expression of Cx45 transcript is observed in the forebrain.
forelimb bud
strong strong
Strong expression of Cx45 transcript is observed in the forelimb and hindlimb buds.
hindlimb bud
strong strong
Strong expression of Cx45 transcript is observed in the forelimb and hindlimb buds.
dorsal aorta
detected detected
There is labeling of the paired dorsal aortae.
embryo ectoderm
weak weak
Labeling of the ectoderm has substantially weakened compared with the previous developmental stages.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gjc1 probeA
Entity Detected:Gjc1, gap junction protein, gamma 1 ( MGI:95718)
Sequence:sense strand is shown

>Gjc1 probeA
TCTAGACCCACTGAAAAGACCATCTTCCTTCTGATAATGTATGGTGTCACAGGCCTCTGCCTATTGCTTA
ACATTTGGGAGATGCTTCACTTAGGGTTTGGGACAATTCGAGACTCACTAAACAGTAAAAGGAGGGAACT
TGATGATCCGGGTGCTTATAATTATCCTTTCACTTGGAACACACCCTCTGCTCCCCCTGGCTATAACATT
GCTGTCAAACCAGATCAGATCCAGTACACTGAGCTGTCCAATGCTAAGATTGCCTACAAGCAAAACAAAG
CCAATATTGCCCAGGAACAGCAGTACGGCAGCCACGAGGAACACCTCCCGGCTGATCTGGAGACTCTGCA
GCGGGAGATCAGAATGGCTCAGGAACGATTGGACCTAGCAATCCAGGCCTACCATCACCAAAACAACCCC
CATGGTCCTCGGGAAAAGAAGGCCAAAGTGGGGTCCAAATCTGGGTCCAACAAAAGCAGTATTAGTAGCA
AATCAGGGGATGGGAAGACCTCCGTCTGGATTTAATCTTGGTTGGGCTTAAAACTTGGGTTTTCATAGTT
TATGGTAAGCAGCAACTTGCTGAATAATGACTTCCATTGAGTAAACATTTGGCTCTGGTTATCTTCAGGG
ATGCTGTTGGCTCATGATCCAAACTCAGGGGACTCTGAAGGTGGAGCTGGGATGAGTCGGGAGAGGGAAA
CACAGTGTTCCCAGGCACATGTTCTCAGCAATAATGCAGTTGCAGAACTTTCAGGTTGTGTCTTCCAGAT
CCAGAGAAGAACTGATATATTTAAATCATTCTTGTTGGACAGTTTTTGTATGTACAGTATTATGGTACAG
TTTTTTTAGTATGATAAATTTTTTTTTTGTATTTGTACATTGGACTGCTGTAGTTACACTTTTTTATATT
AAAGGAAAAAATCCTTGTAAATAAAAAAAAAAAAAAAAAAAA
nt 1122 - nt 2073 of NM_008122.2
Notes:The Gjc1 (Cx45) probe used in this study by Alcolea et al., 1999 [PMID:10381888] is described as follows: "The 0.95-kb Cx45 antisense probe was synthesized with T7 RNA-polymerase from the XbaI-linearized plasmid. The probe matched 521 bp of the C-terminal coding region of the mouse Cx45 gene plus 429 bp of the 3'-untranslated region."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Alcolea S; Theveniau-Ruissy M; Jarry-Guichard T; Marics I; Tzouanacou E; Chauvin JP; Briand JP; Moorman AF; Lamers WH; Gros DB, 1999 [PMID:10381888] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10381888] Alcolea S, Theveniau-Ruissy M, Jarry-Guichard T, Marics I, Tzouanacou E, Chauvin JP, Briand JP, Moorman AF, Lamers WH, Gros DB 1999 Downregulation of connexin 45 gene products during mouse heart development. Circ Res (1999):1365-79
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE