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EMAGE:5868

Adamts5 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) ( MGI:1346321)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5868
e9.5 WISH clone577, [doi:10.1038/nature01270] Gitton et al., 2002 Nature 2002 "A gene expression map of human chromosome 21 orthologues in the mouse." Dec 5;420(6915):586-90 [PMID:12466855] © Nature Publishing Group. Reproduced with permission from Nature.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:5868Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5868_wholemount__moderate.wlz
5868_wholemount__weak.wlz
5868_wholemount__notDetected.wlz
5868_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5868_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
tail unsegmented mesenchyme
detected detected
limb
detected detected
future brain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1005239
Entity Detected:Adamts5, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2) ( MGI:1346321)
Sequence:sense strand is shown

>MGI:1005239
AAAAAGCCACTCCTGCGTGGGTCCTGGGCAGAGTATGAACGAATTTATGGGGATGGATCTTCCCGCATCC
TGTATGTCTACAACCGCGAGGGCTTTAGCTTCGAGGCCCTGCCGCCACGCGCCAGTTGCGAGACTCCTGC
ATCCCCATCTGGGCCCCAAGAGAGCCCCTCGGTGCACAGTAGATCTAGGAGACGCTCAGCGCTGGCCCCG
CAGCTGCTGGACCACTCAGCTTTCTCGCCATCTGGGAACGCGGGACCTCAGACTTGGTGGAGGCGTAGGC
GCCGTTCCATCTCCAGGGCCCGCCAGGTGGAGCTCCTCTTGGTGGCTGACTCGTCCATGGCCAGGATGTA
TGGGCGGGGCCTGCAGCATTACCTGCTGACCCTGGCCTCCATCGCCAACAGGCTGTACAGTCATGCAAGC
ATTGAGAACCACATCCGCCTGGCGGTGGTGAAGGTGGTGGTGCTGACGGACAAGGACACGAGTCTGGAGG
TGAGCAAGAATGCGGCCACGACCCTCAAGAACTTTTGCAAGTGGCAGCACCAACATAACCAGCTAGGGGA
TGATCACGAAGAGCACTACGATGCAGCCATCCTGTTCACCCGAGAGGAT
Notes:The Adamts5 probe used in this study by Gitton et al., 2002 [PMID:12466855] is described as "synthesised by in vitro transcription from 1-2 ug of PCR-amplified cDNAs (insert size range of 500 - 1,500 bp)." Editors note: The clone used to derive this probe is specified on the project website http://chr21.molgen.mpg.de/hsa21/index.html as clone#577 (IMAGp998L045419 from RZPD).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Gitton Y; Dahmane N; Baik S; Ruiz I Altaba A; Neidhardt L; Scholze M; Herrmann BG; Kahlem P; Benkahla A; Schrinner S; Yildirimman R; Herwig R; Lehrach H; Yaspo ML, 2002 [PMID:12466855] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1038/nature01270] [ PMID:12466855] Gitton Y, Dahmane N, Baik S, Ruiz i Altaba A, Neidhardt L, Scholze M, Herrmann BG, Kahlem P, Benkahla A, Schrinner S, Yildirimman R, Herwig R, Lehrach H, Yaspo ML 2002 A gene expression map of human chromosome 21 orthologues in the mouse. Nature (420):586-90
Links:MGI:2674661 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI