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EMAGE:5898

Lpar1 lysophosphatidic acid receptor 1 ( MGI:108429)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5898
Figure 3A. [doi:10.1002/dvdy.21736] Copyright. This image is from Ohuchi H; Hamada A; Matsuda H; Takagi A; Tanaka M; Aoki J; Arai H; Noji S, "Expression patterns of the lysophospholipid receptor genes during mouse early development." Dev Dyn 2008; 237: 3280-94. Reprinted with permission of Wiley-Liss Inc. [PMID:18924241] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: ba1, first branchial arch; ba2, second branchial arch; fl, forelimb bud; hl, hindlimb bud; mx, maxillary process.
Expression Pattern Description
Spatial Annotation:
EMAGE:5898Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5898_wholemount__moderate.wlz
5898_wholemount__weak.wlz
5898_wholemount__notDetected.wlz
5898_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5898_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch maxillary component
strong strong
2nd branchial arch
strong strong
neural tube
strong strong
trunk somite
detected detected
regional
tail unsegmented mesenchyme
strong strong
regionalLpa1 is expressed in the presomitic mesoderm but not in the most anterior part of the presomitic mesoderm.
limb
strong strong
head mesenchyme
detected detected
regionalHead mesenchyme at the caudal midbrain and rostral hindbrain was positive for Lpar1 mRNA.
cardiovascular system
detected detected
regionalExpressed in blood vessels.
nasal process
detected detected
tail neural tube
detected detected
regionalExpressed in the floorplate of the caudal neural tube
1st branchial arch
strong strong
tail somite
strong strong
In the somites, the most intense signals were observed in the caudal region of the body.
ear
detected detected
Expressed in periotic region
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lpar1 probeA
Entity Detected:Lpar1, lysophosphatidic acid receptor 1 ( MGI:108429)
Sequence:sense strand is shown

>Lpar1 probeA
GCAGCTGCCTCTACTTCCAGCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAACGAACAACAGTGCT
TCTACAATGAGTCTATCGCCTTCTTTTATAACCGGAGTGGGAAATATCTAGCCACAGAATGGAACACAGT
GAGCAAGCTGGTGATGGGACTGGGCATCACTGTTTGCGTGTTCATCATGTTGGCCAATCTCCTGGTCATG
GTGGCAATCTACGTCAACCGCCGCTTCCATTTCCCTATTTATTACTTGATGGCCAACCTGGCTGCTGCAG
ACTTCTTCGCTGGATTGGCCTACTTCTACCTGATGTTCAATACAGGACCTAATACCCGGAGACTGACTGT
TAGCACGTGGCTCCTCCGGCAGGGCCTCATTGACACCAGCCTGACAGCTTCTGTGGCCAACCTGCTGGCT
ATTGCTATCGAGAGGCACATCACGGTTTTCCGCATGCAGCTCCATACACGAATGAGCAACCGGCGCGTGG
TGGTGGTGATTGTAGTCATCTGGACTATGGCCATTGTGATGGGTGCTATACCCAGTGTGGGCTGGAACTG
CATCTGTGATATCGATCACTGTTCCAACATGGCACCCCTCTACAGTGACTCCTACTTAGTCTTCTGGGCC
ATTTTCAACCTGGTGACCTTTGTGGTCATGGTGGTTCTCTACGCTCACATCTTTGGCTATGTTCGCCAGA
GGACTATGAGGATGTCTCGGCATAGTTCTGGACCCAGGAGGAATCGGGACACCATGATGAGCCTTCTGAA
GACTGTGGTCATTGTGCTTGGTGCCTTTATTGTCTGCTGGACTCCGGGATTGGTCTTGTTATTGCTGGAT
GTGTGCTGCCCGCAGTGCGATGTCCTGGCCTATGAGAAGTTCTTCCTCCTCCTGGCCGAGTTCAACTCTG
CTATGAACCCCATCATCTACTCCTACCGCGACAAAGAGATGAGCGCCACCTTCAGGCAGATCCTGT
nt 279 - nt 1254 of NM_172989.1
Notes:The Lpar1 (Lpa1) probe used in this study by Ohuchi et al., 2008 [PMID:18924241] (0.976kb) was generated by PCR using the primers 5'-GCAGCTGCCTCTACTTCCAG-3' (left) and 5'-ACAGGATCTGCCTGAAGGTG-3' (right) from template cDNA obtained from Tanaka et al. 2006 [PMID:16829511] .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ohuchi H; Hamada A; Matsuda H; Takagi A; Tanaka M; Aoki J; Arai H; Noji S, 2008 [PMID:18924241] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1002/dvdy.21736] [ PMID:18924241] Ohuchi H, Hamada A, Matsuda H, Takagi A, Tanaka M, Aoki J, Arai H, Noji S 2008 Expression patterns of the lysophospholipid receptor genes during mouse early development. Dev Dyn (237):3280-94
 [ doi:10.1074/jbc.M605142200] [ PMID:16829511] Tanaka M, Okudaira S, Kishi Y, Ohkawa R, Iseki S, Ota M, Noji S, Yatomi Y, Aoki J, Arai H 2006 Autotaxin stabilizes blood vessels and is required for embryonic vasculature by producing lysophosphatidic acid. J Biol Chem (281):25822-30
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE