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EMAGE:5904

S1pr3 sphingosine-1-phosphate receptor 3 ( MGI:1339365)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5904
Figure 3H. [doi:10.1002/dvdy.21736] Copyright. This image is from Ohuchi H; Hamada A; Matsuda H; Takagi A; Tanaka M; Aoki J; Arai H; Noji S, "Expression patterns of the lysophospholipid receptor genes during mouse early development." Dev Dyn 2008; 237: 3280-94. Reprinted with permission of Wiley-Liss Inc. [PMID:18924241] .

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: te, telencephalon. Inset shows more intense expression in somites in caudal region.
Expression Pattern Description
Spatial Annotation:
EMAGE:5904Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5904_wholemount__moderate.wlz
5904_wholemount__weak.wlz
5904_wholemount__possible.wlz
5904_wholemount__notDetected.wlz
5904_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5904_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
head mesenchyme
detected detected
1st branchial arch
detected detected
limb
detected detected
embryo
detected detected
regionalExpressed in facial processes.
trunk somite
detected detected
regionalMore intense signals for S1p3 were observed in somites in the caudal region of the embryo.
tail somite
strong strong
Expression in somites is more intense in the caudal region
nasal process
detected detected
Expressed in facial processes
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:S1pr3 probeA
Entity Detected:S1pr3, sphingosine-1-phosphate receptor 3 ( MGI:1339365)
Sequence:sense strand is shown

>S1pr3 probeA
CACCCTCATCACCACCATCCTCTTCTTGGTCACCTGTAGCTTCATCGTCTTGGAGAACCTGATGGTTTTG
ATTGCCATCTGGAAAAACAATAAATTTCACAACCGCATGTACTTTTTCATCGGCAACTTGGCTCTCTGCG
ACCTGCTGGCCGGCATAGCATACAAGGTCAATATTCTTATGTCCGGCAGGAAGACGTTCAGTCTGTCTCC
AACAGTGTGGTTCCTCAGGGAGGGCAGTATGTTCGTAGCCCTGGGCGCATCCACCTGCAGCTTACTGGCC
ATTGCCATTGAGCGACACCTGACCATGATCAAGATGAGGCCATATGATGCCAACAAGAAGCACCGCGTGT
TCCTTCTGATTGGGATGTGCTGGCTAATTGCCTTCTCGCTGGGTGCCCTGCCAATCCTGGGCTGGAACTG
CCTGGAGAACTTTCCCGACTGCTCTACCATCTTGCCCCTCTACTCCAAGAAATACATCGCCTTCCTCATC
AGTATCTTCACCGCCATTCTGGTGACCATCGTCATCTTGTATGCGCGCATCTACTGCCTGGTCAAGTCCA
GCAGCCGCAGGGTGGCCAACCACAACTCTGAGAGATCCATGGCCCTTCTGCGGACCGTAGTGATTGTGGT
GAGTGTGTTCATTGCCTGTTGGTCCCCACTTTTTATCCTCTTCCTCATCGACGTGGCCTGCAGGGCAAAG
GAGTGCTCCATCCTCTTCAAGAGTCAGTGGTTCATCATGCTGGCTGTCCTCAACTCGGCCATGAACCCTG
TCATCTACACGCTGGCCAGCAAAGAGATGAGGCGCGCCTTCTTCCGGTTGGTGTGCGGCTGTCTAGTCAA
GGGCAAGGGGACCCAGGCCTCACCCATGCAGCCTGCCCTCGACCCAAGCAGAAGTAAGTCAAGCTCCAGT
AACAACAGCAGCCACTCTCCGAAGGTCAAGGAAGACCTG
nt 442 - nt 1390 of NM_010101.3
Notes:The S1pr3 (S1p3) probe used in this study by Ohuchi et al., 2008 [PMID:18924241] was generated by PCR using the primers 5'-CACCCTCATCACCACCATCCTCT-3' (left) and 5'-CAGGTCTTCCTTGACCTTCG-3' (right) and a template cDNA from "Hitachi cDNA library". The probe transcribed from it is 949nt in length. NM_010101 is given as an identifier.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ohuchi H; Hamada A; Matsuda H; Takagi A; Tanaka M; Aoki J; Arai H; Noji S, 2008 [PMID:18924241] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1002/dvdy.21736] [ PMID:18924241] Ohuchi H, Hamada A, Matsuda H, Takagi A, Tanaka M, Aoki J, Arai H, Noji S 2008 Expression patterns of the lysophospholipid receptor genes during mouse early development. Dev Dyn (237):3280-94
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE